83330

Pyruvate Kinase from rabbit muscle

crystalline suspension in 3.8 M ammonium sulfate solution, ~350 U/mg protein (10-40 mg/ml)

• Sinônimo(s): ATP:pyruvate 2-O-phosphotransferase, PK
• Número CAS: 9001-59-6
• Número da licença da enzima: 2.7.1.40 (BRENDA, IUBMB)
• Número CE: 232-616-2
• Número MDL: MFCD00081946
• Código UNSPSC: 12352204
• eCl@ss: 32160410
• NACRES: NA.54

Propriedades

• Formulário: suspension
• qualidade: crystalline suspension in 3.8 M ammonium sulfate solution
• atividade específica: ~350 U/mg protein (10-40 mg/ml)
• peso molecular: M_r ~230000
• pH: ~6
• atividade externa: lactate dehydrogenase ≤0.01%
• temperatura de armazenamento: 2-8°C

Descrição

Ações bioquímicas/fisiológicas

Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa.
Isoelectric Point: 7.6
Optimal pH: ∼7.5
Optimal Temperature: 25°C
ΕA₂₈₀ = 0.54 for 1 mg(p)/ml, 1 cm path
Reported K_M values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.

Pyruvate kinase catalyzes the conversion of phosphoenolpyruvate to pyruvate, whitch is the rate-limiting final step in glycolysis.

Definição da unidade

1 U corresponds to the amount of enzyme which catalyzes the transphosphorylation of 1 μmol PEP per min at pH 7.4 and 25 °C

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)