79454

Ampliflu^™ Red Western Blot Kit

for fluorescent detection

• Código UNSPSC: 12352200

Propriedades

• uso: sufficient for 50 applications
• fluorescência: λ_ex 571 nm; λ_em 585 nm
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

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Aplicação

The Ampliflu Red Western Blot Kit provides an alternative to the classical chemiluminescence detection. When working with Ampliflu Red, standard protocols for the immunoblotting-procedure can be used until the detection step. No extra time for signal accumulation is necessary as it is for chemiluminescent signals. The use of Ampliflu Red is suitable for protein quantities between 1 ng and 1 μg per band of protein.

Princípio

The kit contains the peroxidase substrate Ampliflu Red that is enzymatically converted to the highly fluorescent compound, resorufin. Fluorescent imaging is then performed on the immunoblot.

Nota de análise

Sensitivity: Limit of detection is 1 ng/band, but may vary depending on the antibodies used.

Informações legais

Ampliflu is a trademark of Sigma-Aldrich Co. LLC

Somente componentes do kit

• Dimethyl sulfoxide, puriss. p.a., ACS reagent, ≥99.9% (GC) 3 mL

Componentes do kit também disponíveis separadamente

• 90101 Ampliflu^™ Red, for fluorescence, ≥98.0% (HPLC) 5 vial(s)
• 79382 Phosphate buffered saline, tablet 5 tablets

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H302
• Declarações de precaução: P264 - P270 - P301 + P312 - P501
• Classificações de perigo: Acute Tox. 4 Oral
• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable