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Sigma-Aldrich

Osmium tetroxide solution

suitable for electron microscopy, 2% in H2O

Sinônimo(s):

Osmium(IV) oxide

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About This Item

Fórmula linear:
OsO4
Número CAS:
20816-12-0
Peso molecular:
254.23
Número MDL:
MFCD00011150
Código UNSPSC:
12171500
ID de substância PubChem:
57652577
NACRES:
NA.47

forma

solution

adequação da reação

reagent type: oxidant

concentração

2% in H2O

técnica(s)

electron microscopy: suitable

cor

colorless to yellow

densidade

1.0 g/mL at 20 °C
1.0 g/mL at 20 °C

aplicação(ões)

hematology
histology

temperatura de armazenamento

2-8°C

cadeia de caracteres SMILES

O=[Os](=O)(=O)=O

InChI

1S/4O.Os

chave InChI

VUVGYHUDAICLFK-UHFFFAOYSA-N

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Descrição geral

Osmium tetroxide (OsO4) also referred to as osmium, is a heavy tetrahedral, symmetrical molecule. It is both a dye and a non-coagulant fixative agent in electron microscopy primarily used for the examination of biological tissue components in the histopathology laboratory. Specimens are postfixed with osmium tetroxide to ensure the preservation of the lipids.

Aplicação

Osmium tetroxide solution has been used as a fixative in electron microscopy.

Ações bioquímicas/fisiológicas

Osmium tetroxide is also referred to as osmium and is mainly used as a secondary fixative of tissues. The molecule reacts with lipid moieties and is responsible for the oxidation of unsaturated bonds of fatty acids. This reaction adds density and contrast to biological samples. In normal tissues, presence of osmium results in intense black staining which can be viewed by normal eye. In addition, it can be used as a mordant (a molecule which combines with stain or dye at later stages) in electron microscopy, thereby enhancing lead staining.

Pictogramas

Skull and crossbonesCorrosion
GHS06,GHS05

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 2 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

Código de classe de armazenamento

6.1B - Non-combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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