SBR00028

FITC labeled vancomycin

for fluorescent microbial imaging

• Sinônimo(s): Vancomycin -FITC, Vancomycin FITC conjugate
• Fórmula empírica (Notação de Hill): C₈₇H₈₈Cl₂N₁₀O₂₉S
• Peso molecular: 1840.65
• Código UNSPSC: 51102829
• NACRES: NA.76

Propriedades

• Nível de qualidade: 100
• Ensaio: ≥85% (HPLC)
• forma: solid
• peso molecular: M_w 1840.66 g/mol
• disponibilidade: not available in China
• condição de armazenamento: protect from light
• cor: yellow to dark orange
• adequação: suitable for (Fluorescent detection of gram-positive bacteria); suitable for (Microbiome); suitable for microbiology
• espectro de atividade do antibiótico: Gram-positive bacteria
• Modo de ação: DNA synthesis
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

FITC labeled Vancomycin (Vancomycin-FITC), is a Fluorescein conjugated Vancomycin, with a similar mode of action as Vancomycin. Vancomycin -FITC can be used to detect Gram-positive bacteria by common fluorescent instruments. Vancomycin is a glycopeptide antibiotic, used to treat gram-positive infections. Fluorescent antibiotics are obtained by synthetic conjugation of an antibiotic to a fluorophore.

Aplicação

Fluorescent antibiotics can be used for many applications including:

• Antimicrobial resistance research.
• Bacterial visualization and imaging.
• Parent antibiotic mode of action research and new antibiotic discovery.
• Toxicity studies.
• Research of bacterial infections and tracking its uptake in vivo.

Ações bioquímicas/fisiológicas

Vancomycin inhibits cell wall synthesis at the level of peptidoglycan biosynthesis. Vancomycin binds with high affinity and specificity to the terminal D-Ala-D-Ala of the growing peptide chain during cell wall synthesis. The binding inhibits transpeptidase activity, which prevents further elongation and cross-linking of the peptidoglycan matrix. Vancomycin-FITC has fluorescein attached to the amine group on the disaccharide moiety of the vancomycin molecule. This disaccharide moiety does not play any role in D-Ala-D-Ala binding site, affording a comparable binding affinity of Vancomycin-FITC to Gram-positive bacteria as the parent drug. However, a minimum inhibitory concentration (MIC) decrease of about two orders of magnitude in comparison to Vancomycin was observed. This activity decrease can be linked to repulsive interactions between the negatively charged Fluorescein moiety and the anionic environment of the peptidoglycan.

Características e benefícios

• High-quality antibiotic suitable for multiple research applications
• Ideal for immunological, biochemical, and cell biology applications
• Suitable for fluorescent microscopy imaging

Nota de análise

• Aliquots of the DMSO solution can be stored at -20°C, protected from light for at least 1 month.
• Fluorescent microscopy application: Vancomycin -FITC has excitation/emission wavelength at 495 nm/515 nm.
• It is recommended to dissolve Vancomycin-FITC in DMSO (Dimethyl Sulfoxide) to a concentration of 1mg/mL.
• Recommended working concentration for Bacillus cereus in fluorescent microscopy imaging application is 25μg/mL.

Outras notas

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Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable