PPB023

Tris buffer

Pre-set, pH 8.1, pHast Pack^™, powder

• Sinônimo(s): 0.1M Tris
• Código UNSPSC: 12161700
• NACRES: NA.25

Propriedades

• Nível de qualidade: 100
• linha de produto: pHast Pack^™
• forma: powder
• condição de armazenamento: (Tightly closed. Dry)
• Impurezas: ≤5 ppm Heavy Metals (lead)
• cor: white
• pH: 8.1
• traços de cátion: Fe: ≤10 ppm
• atividade externa: DNase, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• temperatura de armazenamento: room temp

Descrição

Descrição geral

Tris buffer, pH 8.1 is a general biological buffer commonly used in biochemistry and molecular biology. It is isotonic and non-toxic in nature due to the presence of tris base. Tris has a buffering capacity range of pH 7.0 - 9.0, with a pKa of 8.1 at 25°C.

Aplicação

Intended for use in:

• Cell culture (sterile filter recommended)
• Western blot
• Protein electrophoresis
• DNA agarose electrophoresis as a component to make Tris acetate-EDTA (TAE) and Tris borate-EDTA (TBE) buffers

Características e benefícios

• Ready-to-use for quick buffer prep
• No measuring and adjusting pH
• Saves time and minimizes effort in the lab
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Embalagem

Foil pouches

Reconstituição

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 0.1M Tris solution, pH 8.1 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture and electrophoresis applications.

Armazenamento e estabilidade

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Outras notas

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Informações legais

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1