PPB022

Tris buffer

Pre-set, pH 7.0, pHast Pack^™, powder

• Sinônimo(s): 0.1M Tris
• Código UNSPSC: 12161700
• NACRES: NA.25

Propriedades

• Nível de qualidade: 100
• linha de produto: pHast Pack^™
• forma: powder
• condição de armazenamento: (Tightly closed. Dry)
• Impurezas: ≤5 ppm Heavy Metals (lead)
• cor: white
• pH: 7.0
• solubilidade: soluble, clear, colorless
• traços de cátion: Fe: ≤10 ppm
• atividade externa: DNase, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• temperatura de armazenamento: room temp

Descrição

Descrição geral

Tris buffer, pH 7.0 is a general buffer that is isotonic and non-toxic in nature due to the presence of tris base. It is suitable for most biochemical and molecular biology applications. Tris has a buffering capacity range of pH 7.0 - 9.0, with a pKa of 8.1 at 25°C.

Aplicação

Tris buffer is used in:

• Western blot
• Protein gel electrophoresis
• cell culture (sterile filter buffer)
• As a buffer component

Características e benefícios

• Fast, ready-to-use buffer
• No need to measure and adjust the pH
• Saves time and minimizes effort in the lab
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Embalagem

Foil pouches

Reconstituição

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 0.1M Tris solution, pH 7.0 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture and electrophoresis applications.

Armazenamento e estabilidade

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Outras notas

For additional information on our range of Biochemicals, please complete this form.

Informações legais

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1