M8884

Methyl Green

~85% purity, powder

• Sinônimo(s): Ethyl Green
• Fórmula linear: C₂₇H₃₅BrClN₃ · ZnCl₂
• Número CAS: 7114-03-6
• Peso molecular: 653.24
• Número do índice de cores: 42590
• Beilstein: 3914106
• Número CE: 230-415-4
• Número MDL: MFCD00151094
• Código UNSPSC: 12171500
• ID de substância PubChem: 24897293
• NACRES: NA.47

Propriedades

• Nome do produto: Methyl Green, zinc chloride salt, ~85%
• Nível de qualidade: 200
• Ensaio: ~85%
• Formulário: powder
• composição: Dye Content, ≥83%
• técnica(s): titration: suitable
• cor: red to very dark red
• pH: 0.2-1.8, yellow to blue
• pf: >300 °C (lit.)
• solubilidade: water: 1 mg/mL
• εmax: >11100.00 at 417-425 nm in water at 0.005 g/L; >16000 at 311-319 nm in water at 0.005 g/L; >60000 at 626-634 nm in water at 0.005 g/L
• aplicação(ões): diagnostic assay manufacturing; hematology; histology
• temperatura de armazenamento: room temp
• cadeia de caracteres SMILES: [Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](\C)C
• InChI: 1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4
• chave InChI: RRKSQWOQZFIQKW-UHFFFAOYSA-J

Descrição

Descrição geral

Methyl green is a synthetic, triphenylmethane-type cationic dye related to ethyl green mainly used as DNA stain in histochemistry. It possesses aniline rings with different degrees of methylation. The dye binds specifically to the A-T rich regions of the major groove of DNA with the help of two positive charges. Methyl green in combination with pyronin is extensively used to stain RNA and plasma cells in tissue sections and cytologic preparations.

Aplicação

Methyl green is suitable as a nuclear counterstain in enzyme histochemistry, immunostaining, and in situ hybridization as the bluish-green color will be in contrast with red, purple, brown, and black products of histochemical reactions. It is also suitable as a tracking dye in Acid-polyacrylamide gel electrophoresis to separate gliadins. It has been used in the histochemical staining for the apoptosis examination of kidney tissue and as a nuclear stain for labeling embryos.

Qualidade

Material may be a mixture of monobromo-monochloro and dichloro ZnCl₂ salts. Halogen contents listed on label.

Nota de análise

Dye content based on molecular weight of monobromo-monochloro ZnCl₂ salt (653.2).

Informações de segurança

• Pictogramas: GHS05,GHS07,GHS09
• Palavra indicadora: Danger
• Frases de perigo: H314,H335,H411
• Declarações de precaução: P260 - P271 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338
• Classificações de perigo: Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3
• Órgãos-alvo: Respiratory system
• Código de classe de armazenamento: 8A - Combustible corrosive hazardous materials
• Classe de risco de água (WGK): WGK 3
• Equipamento de proteção individual: dust mask type N95 (US), Eyeshields, Gloves