E9379

Stains-All

~95% purity, powder

• Sinônimo(s): 1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphtho[1,2-d]thiazolium bromide, 3,3′-Diethyl-9-methyl-4,5,4′,5′-dibenzothiacarbocyanine
• Fórmula empírica (Notação de Hill): C₃₀H₂₇BrN₂S₂
• Número CAS: 7423-31-6
• Peso molecular: 559.58
• Beilstein: 3865213
• Número CE: 231-047-7
• Número MDL: MFCD00078788
• Código UNSPSC: 12171500
• ID de substância PubChem: 24894694
• NACRES: NA.47

Propriedades

• product name: Stains-All, ~95%
• Ensaio: ~95%
• Nível de qualidade: 200
• forma: powder
• solubilidade: chloroform: 40 mg (PLUS 4.0 ml)
• aplicação(ões): diagnostic assay manufacturing; hematology; histology
• temperatura de armazenamento: 2-8°C
• cadeia de caracteres SMILES: [Br-].CCN1C(/Sc2ccc3ccccc3c12)=C\C(C)=C\c4sc5ccc6ccccc6c5[n+]4CC
• InChI: 1S/C30H27N2S2.BrH/c1-4-31-27(33-25-16-14-21-10-6-8-12-23(21)29(25)31)18-20(3)19-28-32(5-2)30-24-13-9-7-11-22(24)15-17-26(30)34-28;/h6-19H,4-5H2,1-3H3;1H/q+1;/p-1
• chave InChI: MPBRYMWMMKKRGC-UHFFFAOYSA-M

Descrição

Aplicação

Stains-All has been used for the staining of gel made for size determination of hyaluronan. It has also been used for the staining of DNA oligonucleotides separated by nondenaturing polyacrylamide gel electrophoresis.

Ações bioquímicas/fisiológicas

Stains-All is a cationic carbocyanine dye and is a multipurpose dye. It stains sialoglycoproteins blue, Ca²⁺ binding proteins deep blue to violet, proteins red and lipids yellow-orange. It also stains RNA (bluish purple) and DNA (blue).

As little as 3 ng (123 BP fragment) of pBR322/Hae III digest DNA and 90 ng tRNA can be detected on a polyacrylamide gel. PAGE gels are stained in the dark and then destaining by removing the gel from the staining solution and exposing it to the light from a light box until sufficient destaining has occurred. A staining solution is typically made by dissolving the dye in formamide and buffer.

Atenção

Upon continued exposure to light the nucleic acid-dye complex will fade and disappear.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)