902020

Desthiobiotin-PEG3-Azide

• Sinônimo(s): (4S,5R)-N-[2-[2-[2-(2-Azidoethoxy)ethoxy]ethoxy]ethyl]-5-methyl-2-oxo-4-imidazolidinehexanamide
• Fórmula empírica (Notação de Hill): C₁₈H₃₄N₆O₅
• Número CAS: 1951424-99-9
• Peso molecular: 414.50
• Número MDL: MFCD28334534
• Código UNSPSC: 51171641
• NACRES: NA.22

Propriedades

• Formulário: paste
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: [N+](=[N-])=NCCOCCOCCOCCNC(=O)CCCCC[C@@H]1NC(=O)N[C@@H]1C
• InChI: 1S/C18H34N6O5/c1-15-16(23-18(26)22-15)5-3-2-4-6-17(25)20-7-9-27-11-13-29-14-12-28-10-8-21-24-19/h15-16H,2-14H2,1H3,(H,20,25)(H2,22,23,26)/t15-,16+/m1/s1
• chave InChI: JIQVKRRASYKCEL-CVEARBPZSA-N

Descrição

Aplicação

Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 10¹¹ vs. 10¹⁵ M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable