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Key Documents

SFLRAB

Roche

KAPA SYBR® FAST

suitable for qPCR, 2 ×, ROX Low

Sinônimo(s):

PCR

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About This Item

Código UNSPSC:
41106300
NACRES:
NA.55

Nível de qualidade

uso

sufficient for 100 reactions
sufficient for 1000 reactions
sufficient for 500 reactions
sufficient for 5000 reactions

prazo de validade

≤12 mo.

Características

dNTPs included
hotstart

embalagem

kit of 1 mL (100 x 20 μL rxn; KK4619)
kit of 10 mL (1000 x 20 μL rxn; KK4621)
kit of 5 mL (500 x 20 μL rxn; KK4620)
kit of 50 mL (5000 x 20 μL rxn; KK4622)

fabricante/nome comercial

Roche

concentração

2 ×

técnica(s)

qPCR: suitable

entrada

purified DNA

compatibilidade

for use with Agilent Mx3000P
for use with Agilent Mx3005P
for use with Agilent Mx4000
for use with Applied Biosystems® 7500
for use with Applied Biosystems® ViiA 7
for use with QuantStudio

método de detecção

SYBR® Green

temperatura de armazenamento

−20°C

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Descrição geral

KAPA SYBR® FAST qPCR Kits contain the first DNA polymerase engineered via directed evolution to be more tolerant of SYBR Green I dye inhibition.

The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis. KAPA SYBR FAST qPCR Kits, developed to perform optimally in stringent real-time PCR reaction conditions, exhibit dramatic improvements to signal-to-noise ratio (fluorescence), quantification cycle (Cq), linearity, and sensitivity.

Aplicação

KAPA SYBR® FAST has been used for:
  • qRT-PCR
  • RT-PCR
  • Gene expression
  • Gene knockdown validation
  • Microarray validation
  • ChIP analysis
  • Low copy detection
  • Absolute quantification of NGS libraries

Características e benefícios

Quantitate changes in gene expression more accurately
  • High reaction efficiency between 95 - 105% improves accuracy and reproducibility.
  • Unbiased efficiency across a wide range of GC contents and amplicon lengths.

Detect low copy and difficult targets consistently
  • Improved processivity results in earlier Cq scores.
  • Higher fluorescence detection across varying AT- and GC-rich targets.
  • Novel enzyme is resistant to SYBR® inhibition.

Complete real-time PCR runs in just 40 minutes
  • 55% shorter run times with fast cycling protocol.
  • Maintain high performance when switching from standard to fast protocols.

Quick Notes:
  • This kit contains an engineered enzyme optimized for qPCR using SYBR Green I dye chemistry.
  • The 2X master mix contains a proprietary buffer. Together with the novel enzyme, this improves amplification efficiency of both GC- and AT-rich targets.
  • 20 sec initial denaturation at 95°C is sufficient for enzyme activation. When working with complex templates, an initial denaturation of 3 min is recommended.
  • For 3-step cycling, use 20 sec for primer annealing and 1 sec for extension/data acquisition at 72°C.
  • Do not exceed 25 μL reaction volumes.

Qualidade

All kit components are subjected to stringent functional quality control, are free of detectable contaminating exo and endonuclease activity, and meet strict requirements with respect to DNA contamination.

Nota de preparo

Always ensure that components have been fully thawed and thoroughly mixed before use. KAPA SYBR® FAST qPCR Master Mix (2X) may not freeze solidly, even when stored at -20°C.

The SYBR Green I dye contained in KAPA SYBR FAST qPCR Master Mix (2X) and ROX/fluorescein dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity.

KAPA SYBR FAST qPCR Master Mix (2X) is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20°C when not in use. When protected from light, reagents are stable in the dark at 4°C for at least one week and may be stored at this temperature for short-term use, provided that they do not become contaminated with microbes and/or nucleases.

Outras notas

For Research Use Only. Not for use in diagnostic procedures.

Informações legais

Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
QuantStudio is a trademark of IROA Technologies LLC
ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SYBR is a registered trademark of Life Technologies

Somente componentes do kit

Nº do produto
Descrição

  • KAPA SYBR® FAST DNA Polymerase

  • reaction buffer

  • dNTPs

  • SYBR® Green I dye

  • MgCl2 2.5 mM

Pictogramas

Health hazard

Palavra indicadora

Warning

Frases de perigo

Classificações de perigo

STOT SE 2

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

does not flash

Ponto de fulgor (°C)

does not flash


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Artigos

An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.

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