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11493027001

Roche

IsoStrip™ Mouse Monoclonal Antibody Isotyping Kit

sufficient for 10 tests

Sinônimo(s):

hybridoma subtyping

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About This Item

Código UNSPSC:
23201100

uso

sufficient for 10 tests

Nível de qualidade

100

fabricante/nome comercial

Roche

temperatura de armazenamento

2-8°C

Categorias relacionadas

Descrição geral

IsoStrip Mouse Monoclonal Antibody Isotyping Kit is a simple, three-step kit for the rapid characterization of mouse monoclonal antibodies.

Contents
  • Development Tubes, contain lyophilized latex beads, precoated with anti-mouse-Ig antibodies
  • Isotyping Strips, precoated with subclass- and light-chain-specific anti-mouse-Ig antibodies

Especificidade

Anti-mouse antibodies, bound to the latex beads, will react with any mouse monoclonal antibody regardless of its isotype.Goat anti-mouse antibodies, immobilized to the strip, specifically bind to each of the common mouse antibody isotypes (IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA), and to the light chains.

Specificity for all specific antibodies beside IgML at 1.0 μg/ml: ≥ 2
IgM Lambda, at 1.0 μg/ml: ≥ 1

Aplicação

The kit is used for the rapid identification of class, subclass, and light-chain types of mouse monoclonal antibodies (immunoglobulins).

Características e benefícios

  • Reliable: Yields comparable results to the standard ELISA assay
  • Fast: 5 minutes from start to finish
  • Easy: Only 3 steps
  • Economical: No equipment or additional reagents required
  • Highly specific: No crossreactivity with FBS

Embalagem

1 kit containing 2 components.

Especificações

Assay time: 5 minutes
Sample material: Hybridoma supernatant, purified mouse monoclonal antibodies, ascites

Nota de preparo

Working solution: Dilute Sample
  • Remove the desired number of isotyping strips from the canister. Remove the caps from an equal number of development tubes.
    The tubes may be labeled with a pencil or felt-tipped lab marker for easy identification.
  • Dilute a sample containing the mouse monoclonal antibody in 1% BSA/ phosphate-buffered saline (PBS), pH 7.2 to 7.6.
    Culture supernatant samples should be diluted 1:10 to 1:100. Ascites samples should be diluted 1:20,000. These are recommended dilutions and may vary depending on the concentration of antibody in your sample. In our experience, a monoclonal antibody
    concentration of 0.1 to 1 g/ml of diluted sample gives the best results. 150 ml of this diluted sample will be added to the development tube.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações legais

IsoStrip is a trademark of Roche

Somente componentes do kit

Nº do produto
Descrição
  • Development Tubes, containing lyophilized latex beads, precoated with anti-mouse-Ig antibodies
  • Isotyping Strips, precoated with subclass- and light-chain-specific anti-mouse-Ig antibodies

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

does not flash

Ponto de fulgor (°C)

does not flash

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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