UC0E02
UCOE® Single Expression Hygromycin Vector Set
Sinônimo(s):
UCOE Mammalian Protein Expression Vectors, UCOE Mammalian Protein Expression Plasmids, Ubiquitous Chromatin Opening Element, UCOE02
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Produtos recomendados
Condições de expedição
dry ice
Nível de qualidade
Descrição geral
The Ubiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression. Unlike traditional vectors, UCOE-containing vectors have the capacity to ease the process of isolating cell line clones that express high-levels of recombinant proteins. UCOE sequences promote reproducible and stable high level expression of a linked gene by altering chromatin structure to a transcriptionally permissive state that negates epigenetic-mediated (e.g. DNA methylation) silencing.
CET 1019 HS-hygro-SceI is a mammalian expression plasmid vector containing the mouse Rps3 UCOE upstream of a strong human cytomegalovirus (hCMV) immediate early promoter-enhancer element.
CET 1019 AS-hygro-SceI is a mammalian expression plasmid vector containing the murine Rps3 UCOE upstream of a guinea pig CMV (gpCMV) promoter-enhancer element.
The CET 1019 HS-hygro-SceI and CET 1019 AS-hygro-SceI vectors possess the following features:
THIS PRODUCT IS ONLY AVAILABLE FOR SALE TO ACADEMIC INSTITUTIONS OR NOT-FOR-PROFIT ENTITIES FOR USE UNDER THIS PRODUCT LABEL LICENSE. FOR INFORMATION ON COMMERCIAL LICENSING OF THE PATENTED UCOE TECHNOLOGY, PLEASE CONTACT licensing@emdmillipore.com.
CET 1019 HS-hygro-SceI is a mammalian expression plasmid vector containing the mouse Rps3 UCOE upstream of a strong human cytomegalovirus (hCMV) immediate early promoter-enhancer element.
CET 1019 AS-hygro-SceI is a mammalian expression plasmid vector containing the murine Rps3 UCOE upstream of a guinea pig CMV (gpCMV) promoter-enhancer element.
The CET 1019 HS-hygro-SceI and CET 1019 AS-hygro-SceI vectors possess the following features:
- A multiple cloning site downstream of the UCOE-CMV combinations containing unique restriction enzyme sites to permit easy insertion of any gene of interest (cDNA or genomic)
- A polyadenylation element from the SV40 early region located downstream of the multiple cloning site for appropriate mRNA 3 end formation
- A hygromycin antibiotic resistance gene for selection of stably transfected mammalian cells
- A β-lactamase gene for plasmid selection and propagation in transformed prokaryotic cells in the presence of ampicillin
- An I-SceI homing restriction endonuclease site for linearization of constructs prior to transfection into mammalian cells, which favors integration into the target cell genome via the pre-generated free DNA ends and thus retains the integrity of the UCOE-CMV-transgene cassettes
THIS PRODUCT IS ONLY AVAILABLE FOR SALE TO ACADEMIC INSTITUTIONS OR NOT-FOR-PROFIT ENTITIES FOR USE UNDER THIS PRODUCT LABEL LICENSE. FOR INFORMATION ON COMMERCIAL LICENSING OF THE PATENTED UCOE TECHNOLOGY, PLEASE CONTACT licensing@emdmillipore.com.
Aplicação
Research Category
General Cell Biology
All
General Cell Biology
All
Research Sub Category
All
All
Ubiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression by removing epigenetic related gene silencing.
Componentes
1) 10ug CET 1019 HS-hygro-SceI (+) Vector (CS221288)
2) 10ug CET 1019 AS-hygro-SceI (+) Vector (CS221289)
3) 10ug SC HS-hygro (-) Vector (CS221290)
4) 10ug SC AS-hygro (-) Vector (CS221291)
2) 10ug CET 1019 AS-hygro-SceI (+) Vector (CS221289)
3) 10ug SC HS-hygro (-) Vector (CS221290)
4) 10ug SC AS-hygro (-) Vector (CS221291)
Armazenamento e estabilidade
Plasmids should be stored at -20C. Avoid repeated freeze-thaw events.
Outras notas
Concentration: Please refer to lot specific datasheet.
Informações legais
UCOE is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Código de classe de armazenamento
12 - Non Combustible Liquids
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Genomics, 82(3), 269-279 (2003-08-09)
The genetic elements that are responsible for establishing a transcriptionally competent, open chromatin structure at a region of the genome that consists only of ubiquitously expressed, housekeeping genes are currently unknown. We demonstrate for the first time through functional analysis
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica