SCT277

BioTracker^™ Red pH-Independent Autophagy Live Cell Probe

Human

• Sinônimo(s): autophagosome live cell probe, red AP live cell probe, red autophagosome probe, red autophagy probe
• Código UNSPSC: 41106514
• NACRES: NA.47

Propriedades

• Nome do produto: BioTracker^™ Red pH-Independent Autophagy Live Cell Probe,
• fonte biológica: human
• Nível de qualidade: 100
• embalagem: vial of 5 nmol
• fabricante/nome comercial: Millipore
• técnica(s): cell culture | stem cell: suitable; flow cytometry: suitable
• Condições de expedição: 2-8°C
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

The small fluorescent molecule SCT277 is used to detect autophagosomes and autolysosomes. The presumed mechanism is that the dye is incorporated into the autophagosome during double-membrane formation via structural features, and then emits fluorescence under hydrophobic conditions. The probe emits in the far red range.SPECTRAL CHARACTERISTICS: Excitation λ: 530 nmEmission λ: 720 nm

Características e benefícios

Fluorescence microscopyFlow cytometry

Descrição-alvo

BioTracker Red pH-Independent Autophagy Live Cell Probe detects autophagosomes and autolysosomes. The presumptive mechanism involves incorporation of the probe via structural features into the autophagosome during double-membrane formation. Upon incorporation, the probe emits fluorescence under hydrophobic conditions. The utility of the probe is conferred by molecular properties that include cell permeability, does not require transfection, and enables live cell detection with fluorescence microscopy or other fluorescence capture instruments.

Armazenamento e estabilidade

Store at -20 °C, protected from light.

Outras notas

SCT277 detects autophagosomes and autolysosomes by the likely mechanism of incorporation into the autophagosome during double membrane formation, and emits red fluorescence under hydrophobic conditions. SCT277 is cell permeable, does not require transfection, and enables live cell imaging with fluorescence microscopy and quantitative assay by flow cytometry.Add 50 uL DMSO to tube of SCT277 (5 nmol) and pipet to dissolve to make a 0.1 mM stock solution. Dilute with culture medium to 0.1 - 0.5 uM to make working solution.

Informações legais

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable