S8001U
CpGenome Human Non-Methylated DNA Standard Set
It is intended for use as a negative control in gene methylation studies, such as bisulfite conversion of DNA with the CpGenome Turbo Bisulfite Modification Kit.
Sinônimo(s):
Human Non-Methylated DNA Standard
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About This Item
Código UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.31
Produtos recomendados
Nível de qualidade
reatividade de espécies
human
fabricante/nome comercial
CpGenome
Upstate®
aplicação(ões)
genomic analysis
Condições de expedição
dry ice
Categorias relacionadas
Descrição geral
The CpGenome Human Non-Methylated DNA Standard is purified from HCT116 DKO cells that contain genetic knockouts of DNA methyltransferases, DNMT1 (-/-) and DNMT3b (-/-) , and have less than 5% methylated DNA. It is intended for use as a negative control in gene methylation studies, such as bisulfite conversion of DNA with the CpGenome Turbo Bisulfite Modification Kit (Cat. No. S7847). Bisulfite-modified DNA can be evaluated by methylation-specific PCR (MSP) technology using the CpG WIZ amplification kits.
Materials Provided:
One vial containing 5 µg (20 µL) of CpGenome Human Non-Methylated DNA Standard at a concentration of 250 ng/µL.
Validation:
Methylation-specific PCR (MSP) was performed on the CpGenome Human Non-Methylated DNA after bisulfite modification with the CpGenome Turbo Bisulfite kit (Cat. No. S7847). Three sets of primers from the CpG WIZ BRCA1 amplification kit (Cat. No. S7830) were used in this assay: the U primer set, which anneals to unmethylated bisulfite-modified DNA; M primers, which anneal to methylated bisulfite-modified sequences; and W primers, which anneal to unmethylated or methylated DNA that has not undergone bisulfite modification. Only the U primers generated product for this Standard.
CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ Methylation Products apply to technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.
Materials Provided:
One vial containing 5 µg (20 µL) of CpGenome Human Non-Methylated DNA Standard at a concentration of 250 ng/µL.
Validation:
Methylation-specific PCR (MSP) was performed on the CpGenome Human Non-Methylated DNA after bisulfite modification with the CpGenome Turbo Bisulfite kit (Cat. No. S7847). Three sets of primers from the CpG WIZ BRCA1 amplification kit (Cat. No. S7830) were used in this assay: the U primer set, which anneals to unmethylated bisulfite-modified DNA; M primers, which anneal to methylated bisulfite-modified sequences; and W primers, which anneal to unmethylated or methylated DNA that has not undergone bisulfite modification. Only the U primers generated product for this Standard.
CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ Methylation Products apply to technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.
Informações legais
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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