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RLI1MAG-92K

Millipore

MILLIPLEX® Rat Liver Injury Panel - Toxicity Multiplex Assay

Sinônimo(s):

Luminex® Rat Liver Toxicity Assay, Millipore Rat Liver Toxicity Assay, Rat Liver Injury Multiplex Panel

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About This Item

Código UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.84

descrição

The analytes available for this multiplex kit are: Liver-type arginase 1 (ARG1), aspartate transaminase 1 (GOT1), α-glutathianone S-transferase (GSTα), sorbitol dehydrogenase (SDH), and 5′-Nucleotidase (5′-NT/CD73).

Nível de qualidade

reatividade de espécies

rat

fabricante/nome comercial

Milliplex®

assay range

accuracy: 77-92%
sensitivity: 0.021-4.038 ng/mL
(MinDC+2SD)

standard curve range: 0.02-15.0 ng/mL
(GSTα)

standard curve range: 0.04-30.0 ng/mL
(5′-NT/CD73)

standard curve range: 0.08-60.0 ng/mL
(GOT1)

standard curve range: 0.10-75.0 ng/mL
(ARG1)

standard curve range: 0.62-450.0 ng/mL
(SDH)

técnica(s)

multiplexing: suitable

método de detecção

fluorometric (Luminex xMAP)

Condições de expedição

wet ice

Descrição geral

The MILLIPLEX® Rat Liver Injury Bead Panel (RLI1MAG-92K) contains all the components necessary to simultaneous quantify the following 5 analytes in serum and plasma samples: Liver-Type Arginase 1 (ARG1)*, Aspartate transaminase 1 (GOT1), α-glutathione S-transferase (GSTα)*, Sorbitol Dehydrogenase (SDH)*, 5′-Nucleotidase/CD73 (5′-NT).

*ARG1, GSTα, and SDH are biomarkers listed in the Predictive Safety Testing Consortium (PSTC) project pipeline which have a strong translational role in drug safety testing.

The MILLIPLEX® portfolio provides a valuable research assay to investigate multiple biomarkers of liver injury in rat serum and plasma samples using the Luminex® xMAP® instrument platform. This kit uses a 96-well format, contains a lyophilized standard cocktail, two quality controls and can measure up to 38 serum or plasma samples in duplicate.

Drug toxicity is the leading cause of acute liver failure in the United States. Patients with liver damage generally display elevated amounts of specific liver proteins in serum; these proteins can serve as biomarkers of drug-related liver toxicity. Monitoring these biomarkers can greatly help clinicians avoid drug-induced liver failure. Performing laboratory tests to characterize the side effects of potential therapeutics is an essential part of drug development. The rat is a leading animal model for these pre-clinical toxicity studies. The search for sensitive, organ-specific toxicity biomarkers is complemented by the development of novel assays to measure these critical analytes. Liver-Type Arginase 1 (ARG1), α-glutathione S-transferase (GSTα), and Sorbitol Dehydrogenase (SDH) are biomarkers listed in the Predictive Safety Testing Consortium (PSTC) project pipeline which have a strong translational role in drug safety testing. Aspartate transaminase 1 (GOT1) and 5′-Nucleotidase / CD73 (5′-NT) are traditional biomarkers recognized by both the Food and Drug Administration (FDA) and its European counterpart, the European Medicines Agency (EMA).

Panel Type: Toxicity

Aplicação

  • Analytes: 5′-Nucleotidase (5′-NT/CD73), Liver-Type Arginase 1 (ARG1), Aspartate transaminase 1 (GOT1), α-Glutathione S-Transferase (GSTα), Sorbitol Dehydrogenase (SDH)
  • Recommended Sample Type: Rat serum and plasma
  • Recommended Sample Dilution: 25 μL per well of 1:25 diluted serum or plasma
  • Assay Run Time: 2 hours at room temperature (20-25°C)
  • Research Category: Toxicity

Características e benefícios

Design your multiplex kit by choosing available analytes within this panel.

Componentes

Rat Liver Injury Standard, 1 vial, lyophilized

Rat Liver Injury Quality Control 1, 1 vial, lyophilized

Rat Liver Injury Quality Control 2, 1 vial, lyophilized

Assay Buffer, 1 bottle, 30 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea

Rat Liver Injury Detection Antibodies, 1 bottle, 3.2 mL

Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL

Mixing Bottle, 1 bottle

Sample Plate, 96 well format, 1 plate

2 Foil Plate Sealers

Outras notas

Please contact Technical Service for linearity of dilution.
Sensitivity: Please see kit protocol for individual assay sensitivities.

Informações legais

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Pictogramas

Skull and crossbonesEnvironment

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1

Código de classe de armazenamento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


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