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PC253L

Sigma-Aldrich

Anti-Calbindin D-28K (Ab-1) Rabbit pAb

lyophilized, Calbiochem®

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.43

fonte biológica

rabbit

Nível de qualidade

forma do anticorpo

serum

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

forma

lyophilized

contém

≤0.1% sodium azide as preservative

reatividade de espécies

baboon, monkey, rat, human

fabricante/nome comercial

Calbiochem®

condição de armazenamento

OK to freeze
avoid repeated freeze/thaw cycles

Isotipo

IgG

Condições de expedição

ambient

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... CALB1(793)
rhesus monkey ... Calb1(677721)

Descrição geral

Anti-Calbindin D-28K (Ab-1), rabbit polyclonal, reecognizes the Calbindin D-28K protein in rat hippocampus. It is validated for use in frozen sections and for T cell co-stimulation.
Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes the calbindin D-28K protein.
Recognizes the Calbindin D-28K protein in rat hippocampus tissue.

Imunogênio

Bovine
purified bovine cerebellum calbindin D-28K protein

Aplicação

Frozen Sections (whole mount/vibrotome sections, 1:1000, fluorescence)

Vibrotome Sections (1:5000-1:10,000 for biotin-streptavidin/peroxidase detection, see application references)

Advertência

Toxicity: Standard Handling (A)

forma física

Undiluted serum.

Reconstituição

Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution will contain ≤0.1% sodium azide. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.

Nota de análise

Positive Control
Rat striatum, hippocampus, or cortex

Outras notas

Antibody specificity was examined in the rat striatum, cortex, and hippocampus. Detection of primary antibody with biotin-streptavidin/peroxidase reagents according to manufacturers directions. Antibody should be titrated for optimal results in individual systems.
Conde, F., et al. 1994. J. Comp. Neurol.341, 95.
Heizmann, C.W. 1993. Acta Neurobiol. Exp.53, 15.
Baimbridge, K.G., et al. 1992. Trends Neurosci.15, 303.
Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci.16, 98.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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