MABT1568

Anti-QSOX1 Antibody, clone MAb492.1

• Sinônimo(s): EC number EC:1.8.3.2, Quiescin Q6, Sulfhydryl oxidase 1, hQSOX
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 200
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: MAb492.1, monoclonal
• peso molecular: calculated mol wt 82.6 kDa; observed mol wt ~N/A kDa
• purificado por: using protein G
• reatividade de espécies: human
• embalagem: antibody small pack of 100
• técnica(s): immunocytochemistry: suitable; immunofluorescence: suitable; inhibition assay: suitable
• Isotipo: IgG1κ
• nº de adesão de ID de proteína: NP_001004128
• nº de adesão UniProt: O00391
• temperatura de armazenamento: -10 to -25°C
• Informações sobre genes: human ... QSOX1(5768)

Descrição

Especificidade

Clone MAb492.1 is a mouse monoclonal antibody that detects Sulfhydryl oxidase 1 (QSOX1).

Imunogênio

A recombinant fragment corresponding to 517 amino acids of human QSOX1 protein.

Aplicação

Quality Control Testing

Evaluated by Immunocytochemistry in MDA-MB-231 cells.

Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected QSOX1 in MDA-MB-231 cells.

Tested Applications

Immunocytochemistry Analysis: A representative lot detected QSOX1 in Immunocytochemistry applications (Ilani, T., et al. (2013). Science. 341(6141):74-6).

Immunofluorescence Analysis: A representative lot detected QSOX1 in Immunofluorescence applications (Ilani, T., et al. (2013). Science. 341(6141):74-6).

Inhibition Assay: A representative lot modulated ECM properties and inhibited tumor cell migration. (Ilani, T., et al. (2013). Science.;341(6141):74-6; Feldman, T., et al. (2020). Oncotarget. 11(4):386-398).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descrição-alvo

Sulfhydryl oxidase 1 (UniProt: O00391; also known as EC number EC:1.8.3.2, hQSOX, Quiescin Q6) is encoded by the QSOX1 (also known as QSCN6, UNQ2520/PRO6013) gene (Gene ID: 5768) in human. QSOX1 is a single-pass membrane glycoprotein that is expressed is observed in heart, placenta, lung, liver, skeletal muscle, pancreas. It is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off to produce the mature protein. Its thioredoxin domain is localized in amino acids 36-156. Two main redox-active domains of QSOX1 have been described. The amino terminus interacts with the thiol-containing substrates and the carboxy terminus catalyzes reduction of molecular oxygen. It oxidizes thiols during protein folding, reducing molecular oxygen to hydrogen peroxide. It introduces disulfide bonds into proteins with a high efficiency and prefers protein substrates rather than small mono- and di-thiol containing molecules. It is shown to be essential for proper assembly of extracellular matrix (ECM). Its higher expression is observed in several malignancies, especially breast and pancreatic carcinoma and it is a key enzyme involved in a tumor′s ability to modify the ECM that enables tumors to communicate with and/or modify its environment at the tumor-stroma interface, resulting in invasion and subsequent growth of the tumor. Two isoforms of QSOX1 have been described that are produced by alternative splicing. (Ref.: Lake, DF., and Faigel, DO. (2014). Antioxid. Redox Signal. 21(3); 485-496; Grossman, I., et al. (2012). J. Mol. Biol. 425(22); 4366-4378).

forma física

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

Reconstituição

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Armazenamento e estabilidade

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable