MABS831

Anti-PI3 Kinase Antibody, p85 Antibody, N-SH3 Antibody, clone 1D5.1

clone 1D5.1, from mouse

• Sinônimo(s): Phosphatidylinositol 3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PtdIns-3-kinase regulatory subunit p85-alpha, PI3-kinase subunit p85-alpha, PI3K regulatory subuni
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 1D5.1, monoclonal
• reatividade de espécies: mouse, human
• embalagem: antibody small pack of 25 μg
• técnica(s): western blot: suitable
• Isotipo: IgG2bκ
• nº de adesão NCBI: NP_852664
• nº de adesão UniProt: P27986
• Condições de expedição: ambient
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... PIK3R1(5295)

Descrição

Descrição geral

Phosphatidylinositol 3-kinase regulatory subunit alpha (UniProt: P27986; also known as Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PtdIns-3-kinase regulatory subunit p85-alpha, PI3-kinase subunit p85-alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha) is encoded by the PIK3R1 (also known as GRB1) gene (Gene ID: 5295) in human. PI3K regulatory subunit alpha catalyzes the phosphorylation of PI(4,5)P2 inositol ring at the 3 position to generate PI(3,4,5)P3, a potent second messenger that mediates survival signaling and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Following ligand binding, tyrosine phosphorylation of growth factor receptors creates docking sites for p85 binding (through its SH2 domains), and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. PI3 kinase alpha plays a role in insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Mutations in PIK3R1 gene are known to be associated with insulin resistance.

Especificidade

Clone 1D5.1 detects p85 subunit of human and murine PI-3 kinase alpha. It targets an epitope within 77 amino acids from the N-terminal region.

Imunogênio

GST/His-tagged recombinant fragment corresponding to 77 amino acids from the N-terminal region of the p85 regulatory subunit of human PI 3-kinase alpha.

Aplicação

Anti-PI3 Kinase, p85, N-SH3, clone 1D5.1, Cat. No. MABS831, is a mouse monoclonal antibody that detects human and murine p85 subunit of PI3 kinase. It has been tested for use in Western Blotting.

Research Category
Signaling

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected PI3 Kinase, p85, N-SH3 in 10 µg of human cerebral cortex tissue lysate.

Qualidade

Evaluated by Western Blotting in NIH/3T3 L1 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PI3 Kinase, p85, N-SH3 in 10 µg of NIH/3T3 L1 cell lysate.

Descrição-alvo

~85 kDa observed; 83.60 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

forma física

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1