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MABS82

Sigma-Aldrich

Anti-phospho-p70 S6 Kinase (Thr389) Antibody, clone 10G7.1

clone 10G7.1, from mouse

Sinônimo(s):

Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, 70 kDa ribosomal protein S6 kinase 1, Short name=P70S6K1, p70-S6K 1, Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K-alp

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified antibody

tipo de produto de anticorpo

primary antibodies

clone

10G7.1, monoclonal

reatividade de espécies

human, mouse

técnica(s)

immunocytochemistry: suitable
western blot: suitable

Isotipo

IgG2aκ

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

phosphorylation (pThr389)

Informações sobre genes

human ... RPS6KB1(6198)

Descrição geral

The p70 S6 kinase is a ubiquitous cytoplasmic protein that is activated in response to cytokines, and growth factors including the epidermal growth factor and fibroblast growth factor. It lies downstream of the mTOR/PI3K pathway and is phosphorylated on multiple residues: Thr389, Thr229, Thr421, Ser371, Ser404, Ser411, Ser418, and Ser424. Thr389, Ser371, and Ser404 are located in the linker domain; whereas Thr421, Ser411, Ser418, Ser424 are located in the autoinhibitory domain, and Thr229 is found in the catalytic domain. These sites may be phosphorylated in a hierarchical sequence leading to the activation of the p70 S6 kinase. The p70 S6 kinase phosphorylates the S6 protein on the 40S ribosomal protein (rpS6), and plays a role in multiple processes including regulation of mRNA molecules with 5’-terminal oligopyrimidine tracts, protein synthesis, cell growth, proliferation, and cell survival. The activity of this kinase may be important in diabetes, cancer, and aging.

Especificidade

This antibody recognizes p70 S6 Kinase phosphorylated at (Thr389). This antibody also recognizes isoforms Alpha I and Alpha II.

Imunogênio

Epitope: Phosphorylated (Thr389)
KLH-conjugated linear peptide corresponding to mouse p70 S6 Kinase phosphorylated at (Thr389).

Aplicação

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected p70 S6 Kinase in A431 cells.
Research Category
Signaling
Research Sub Category
Cell Cycle, DNA Replication & Repair
This Anti-phospho-p70 S6 Kinase (Thr389) Antibody, clone 10G7.1 is validated for use in Western Blotting, ICC for the detection of phospho-p70 S6 Kinase (Thr389).

Qualidade

Evaluated by Western Blot in untreated and serum treated NIH/3T3 cell lysates.

Western Blot Analysis: 1 µg/mL of this antibody detected p70 S6 Kinase on 10 µg of untreated and serum treated NIH/3T3 cell lysates.

Descrição-alvo

~70/85 kDa observed. This protein recognizes isoforms Alpha I and Alpha II. Uncharacterized bands appear at ~18 and 200 kDa in some lysates depending on treatment conditions.

Ligação

Replaces: 04-392

forma física

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
Untreated and serum treated NIH/3T3 cell lysates

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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