MABS82
Anti-phospho-p70 S6 Kinase (Thr389) Antibody, clone 10G7.1
clone 10G7.1, from mouse
Sinônimo(s):
Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, 70 kDa ribosomal protein S6 kinase 1, Short name=P70S6K1, p70-S6K 1, Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K-alp
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified antibody
tipo de produto de anticorpo
primary antibodies
clone
10G7.1, monoclonal
reatividade de espécies
human, mouse
técnica(s)
immunocytochemistry: suitable
western blot: suitable
Isotipo
IgG2aκ
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
phosphorylation (pThr389)
Informações sobre genes
human ... RPS6KB1(6198)
Descrição geral
The p70 S6 kinase is a ubiquitous cytoplasmic protein that is activated in response to cytokines, and growth factors including the epidermal growth factor and fibroblast growth factor. It lies downstream of the mTOR/PI3K pathway and is phosphorylated on multiple residues: Thr389, Thr229, Thr421, Ser371, Ser404, Ser411, Ser418, and Ser424. Thr389, Ser371, and Ser404 are located in the linker domain; whereas Thr421, Ser411, Ser418, Ser424 are located in the autoinhibitory domain, and Thr229 is found in the catalytic domain. These sites may be phosphorylated in a hierarchical sequence leading to the activation of the p70 S6 kinase. The p70 S6 kinase phosphorylates the S6 protein on the 40S ribosomal protein (rpS6), and plays a role in multiple processes including regulation of mRNA molecules with 5’-terminal oligopyrimidine tracts, protein synthesis, cell growth, proliferation, and cell survival. The activity of this kinase may be important in diabetes, cancer, and aging.
Especificidade
This antibody recognizes p70 S6 Kinase phosphorylated at (Thr389). This antibody also recognizes isoforms Alpha I and Alpha II.
Imunogênio
Epitope: Phosphorylated (Thr389)
KLH-conjugated linear peptide corresponding to mouse p70 S6 Kinase phosphorylated at (Thr389).
Aplicação
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected p70 S6 Kinase in A431 cells.
Research Category
Signaling
Signaling
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
This Anti-phospho-p70 S6 Kinase (Thr389) Antibody, clone 10G7.1 is validated for use in Western Blotting, ICC for the detection of phospho-p70 S6 Kinase (Thr389).
Qualidade
Evaluated by Western Blot in untreated and serum treated NIH/3T3 cell lysates.
Western Blot Analysis: 1 µg/mL of this antibody detected p70 S6 Kinase on 10 µg of untreated and serum treated NIH/3T3 cell lysates.
Western Blot Analysis: 1 µg/mL of this antibody detected p70 S6 Kinase on 10 µg of untreated and serum treated NIH/3T3 cell lysates.
Descrição-alvo
~70/85 kDa observed. This protein recognizes isoforms Alpha I and Alpha II. Uncharacterized bands appear at ~18 and 200 kDa in some lysates depending on treatment conditions.
Ligação
Replaces: 04-392
forma física
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Armazenamento e estabilidade
Stable for 1 year at 2-8°C from date of receipt.
Nota de análise
Control
Untreated and serum treated NIH/3T3 cell lysates
Untreated and serum treated NIH/3T3 cell lysates
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Não está encontrando o produto certo?
Experimente o nosso Ferramenta de seleção de produtos.
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Yu-Ting Kang et al.
Oncotarget, 8(62), 105536-105552 (2017-12-30)
Autophagy is an intracellular recycling and degradation process for regulating tumor progression, survival and drug resistance. Nickel compounds have been identified as human carcinogens. However, the role of nickel-induced autophagy in lung carcinogenesis has not yet been fully elucidated. In
Jin-Zhu Wang et al.
The Journal of clinical investigation, 132(8) (2022-03-04)
Y-box-binding protein 1 (YB-1) is a multifunctional RNA binding protein involved in virtually every step of RNA metabolism. However, the functions and mechanisms of YB-1 in one of the most aggressive cancers, glioblastoma, are not well understood. In this study
Kristin Dauner et al.
The Journal of biological chemistry, 292(14), 5737-5747 (2017-02-16)
The plasma membrane of mammalian cells undergoes constitutive endocytosis, endocytic sorting, and recycling, which delivers nutrients to the lysosomes. The receptors, along with membrane lipids, are normally returned to the plasma membrane to sustain this action. It is not known
Tingting Sun et al.
Aging, 12(23), 24208-24218 (2020-11-24)
The 5-year survival rate of ovarian cancer patients is only 47%, and developing novel drugs for ovarian cancer is needed. Herein, we evaluated if and how SRT2183, a sirtuin-1 activator, impairs the ovarian cancer cells. OVCAR-3 and A2780 cells were
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica