MABS522

Anti-PIKFYVE Antibody, clone R159.4.3C9

clone R159.4.3C9, from mouse

• Sinônimo(s): 1-phosphatidylinositol 3-phosphate 5-kinase, Epididymis luminal protein 37, FYVE finger-containing phosphoinositide kinase, PIKfyve, Phosphatidylinositol 3-phosphate 5-kinase type III, PIPkin-III, Type III PIP kinase, Zinc finger FYVE domain containing 2
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: R159.4.3C9, monoclonal
• reatividade de espécies: human
• técnica(s): western blot: suitable
• Isotipo: IgG2aκ
• nº de adesão NCBI: NP_055855
• nº de adesão UniProt: Q9Y2I7
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... PIKFYVE(200576)

Descrição

Descrição geral

1-phosphatidylinositol 3-phosphate 5-kinase (EC 2.7.1.150; UniProt Q9Y2I7; also known as Epididymis luminal protein 37, FYVE finger-containing phosphoinositide kinase, PIKfyve, Phosphatidylinositol 3-phosphate 5-kinase type III, PIPkin-III, Type III PIP kinase, Zinc finger FYVE domain containing 29) is encoded by the PIKFYVE (also known as CFD, FAB1, HEL37, KIAA0981, PIP5K, PIP5K3, ZFYVE29) gene (Gene ID 200576) in human. PIKfyve is a class III lipid kinase that is responsible for phosphorylating phosphatidylinositol (PtdIns) and phosphatidylinositol 3-phosphate (PtdIns3P) of their inositol ring at the D-5 position to form PtdIns3P and PtdIns(3,5)P2 (phosphatidylinositol 3,5-bisphosphate), respectively. PIKfyve forms a stable ternary heterooligomeric complex with its regulator ArPIKfyve (encoded by the VAC14 gene) and the Sac1 domain-containing PtdIns(3,5)P2 5-phosphatase Sac3 (encoded by the FIG4 gene), and both ArPIKfyve and Sac3 are required for maximal PIKfyve activity. PIKfyve is also known to interact with the Rab9 effector RABEPK and the kinesin adaptor JLP (encoded by SPAG9). By regulating endosomal trafficking, PIKfyve plays an important role in multiple biological processes, such as GLUT4 translocation, retroviral budding, autophagy, and neurodegeneration. Human PIKfyve is initially produced as a 2098-amino acid protein whose N-terminal initiator methionine is removed posttranslationally. PIKFYVE gene mutation is linked to corneal fleck dystrophy (CFD) characterized by white flecks scattered in all levels of the stroma.

Especificidade

Expected to react with all four spliced isoforms of human PIKfyve reported by UniProt (Q9Y2I7-1 through Q9Y2I7-4).

Imunogênio

AviTag-6xHis-tagged recombinant protein corresponding an internal fragment of human PIKFYVE.

Epitope: Internal (N-terminal half).

Aplicação

Anti-PIKFYVE Antibody, clone R159.4.3C9 is an antibody against PIKFYVE for use in Western Blotting.

Research Category
Signaling

Research Sub Category
General Post-translation Modification

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected PIKFYVE in 10 µg of HepG2 cell lysate.

Qualidade

Evaluated by Western Blotting in HL-60 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected PIKFYVE in 20 µg of HL-60 cell lysate.

Descrição-alvo

~240/50 kDa observed. 237,1 kDa (isoform 1), 50.21 kDa (isoform 2), 51.48 kDa (isoform 3), 61.60 kDa (isoform 4) calculated.

forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable