MABS2307

Anti-JMY Antibody, clone HMY 117

• Sinônimo(s): Junction-mediating and -regulatory protein
• Código UNSPSC: 12352203
• NACRES: NA.43

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 200
• conjugado: unconjugated
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: HMY 117, monoclonal
• peso molecular: calculated mol wt 111.45 kDa; observed mol wt ~130 kDa
• purificado por: using protein G
• reatividade de espécies: human
• embalagem: antibody small pack of 100 μL
• técnica(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotipo: IgG2aκ
• sequência de epítopo: Unknown
• nº de adesão de ID de proteína: NP_689618
• nº de adesão UniProt: Q8N9B5
• Condições de expedição: ambient
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... JMY(133746)

Descrição

Descrição geral

Junction-mediating and -regulatory protein (UniProt: Q8N9B5; also known as JMY) is encoded by the JMY gene (Gene ID: 133746) in human. JMY is involved in a complex pathway connecting cell motility and invasion with the p53 response to DNA damage. It is widely expressed in many types of tissue- with cytoplasmic and nuclear co-expression. It contains a unique N-terminal domain (aa 1-126) that is essential for its interaction with p300/EP300; three central coiled-coil segments (aa 324-360; 489-541; and 590-621), a polyproline region (aa 801-830), and a C-terminal WH2 domain (aa 921-938). It localizes primarily to the nucleus, but is also found at the leading edge in migrating cells. It acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In the nucleus, it acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300 and increases p53/TP53-dependent transcription and apoptosis. In the cytoplasm, it acts as a nucleation-promoting factor for both branched and unbranched actin filaments. It promotes the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. However, it does not affect elongation of preformed filaments. JMY is shown to be expressed in every type of tumor investigated. Overexpression of JMY in human U2OS cells is shown to induce the formation of elongated actin filament structures. JMY is ubiquitinated by MDM2 that leads to its proteasomal degradation. In case of DNA damage its interaction with MDM2 is altered that prevents its degradation and allows interaction with p300/EP300. (Ref.: Adighibe, O., et al. (2014). Virchows Arch. 465(6); 715-722; Firat-Karalar, EN., et al. (2011). Mol. Biol. Cell. 22(23); 4563-4574; Zuchero, JB., et al. (2009). Nat. Cell Biol. 11(4); 451-459).

Especificidade

Clone HMY 117 is a mouse monoclonal antibody that detects Junction-mediating and -regulatory protein (JMY).

Imunogênio

KLH-conjugated three peptides corresponding to 13 amino acids from the N-terminal region, 14 amino acids from the internal region, and 23 amino acids from the C-terminal region of human Junction-mediating and -regulatory protein (JMY).

Aplicação

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Junction-mediating and -regulatory protein (JMY) in HeLa cell lysates.

Tested Applications

Immunohistochemistry Applications: A representative lot detected JMY in Immunohistochemistry applications (Adighibe, O., et al. (2014). Virchows Arch. 465(6):715-22).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-JMY, clone HMY 117, Cat. No. MABS2307, is a mouse monoclonal antibody that detects Junction-mediating and -regulatory protein (JMY) and is tested for use in Immunohistochemistry and Western Blotting.

forma física

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Recommended storage: +2°C to +8°C.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable