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Documentos Principais

MABF264

Sigma-Aldrich

Anti-AIP1/WD repeat-containing protein 1, clone 13F10 Antibody

clone 13F10, from rat

Sinônimo(s):

WD repeat-containing protein 1, Actin-interacting protein 1, AIP1, AIP1/WD repeat-containing protein 1

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rat

Nível de qualidade

300

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

13F10, monoclonal

reatividade de espécies

mouse

técnica(s)

western blot: suitable

Isotipo

IgG2bκ

nº de adesão NCBI

NP_035845

nº de adesão UniProt

O88342

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

mouse ... Wdr1(22388)

Descrição geral

Actin-interacting protein 1 (AIP1), also known as WD repeat-containing protein 1 (Wdr1), is a key player in cytoskeletal rearrangements and actin mobilization, and thus plays a role in many cellular situations from movement to cytokinesis. AIP1 interacts with Cofilin as well, and Cofilin regulates actin filament dynamics by stimulating actin filament disassembly and also plays a critical role in cytokinesis and chemotactic migration. Knockdown of AIP1 in HeLa cells increased the percentage of multinucleate cells and the mechanics of cytokinesis were altered with an excessive buildup of actin filaments at telophase. The results could be relieved by overexpression of Cofilin pointing to AIP1’s critical role in Cofilin-mediated actin filament disassembly. Furthermore, AIP1 inhibition caused abnormal cell migration in response to cytokine stimulation leading to a non-directional migration phenotype thus indicating that AIP1 plays an important directional role in response to stimuli as well. Finally AIP1, as one might expect, plays a critical role in development of cells and tissues, especially in epithelial morphogenesis for instance.

Imunogênio

Recombinant protein corresponding to mouse AIP1/WD repeat-containing protein 1.

Aplicação

This Anti-AIP1/WD repeat-containing protein 1, clone 13F10 Antibody is validated for use in Western Blotting for the detection of AIP1/WD repeat-containing protein 1.
Western Blotting Analysis: A representative lot detected AIP1/WD repeat-containing protein 1 in both endogenous and transfected mouse AIP1 in western blots of cell lysates from J774 cells, 293T cells and 293T cells transfected with HA-tagged mouse Aip1 (Li, J., et al. (2007). Nature. 9(3):276-286).

Qualidade

Evaluated by Western Blotting in RAW264.7 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected AIP1/WD repeat-containing protein 1 in 10 µg of RAW264.7 cell lysate.

Descrição-alvo

~66 kDa observed

forma física

Format: Purified

Outras notas

Concentration: Please refer to lot specific datasheet.

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Código de classe de armazenamento

10 - Combustible liquids

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AIP1 acts with cofilin to control actin dynamics during epithelial morphogenesis.
Chu, D; Pan, H; Wan, P; Wu, J; Luo, J; Zhu, H; Chen, J
Development null
Juying Li et al.
Nature cell biology, 9(3), 276-286 (2007-02-13)
Coordinated regulation of cell migration, cytokine maturation and apoptosis is critical in inflammatory responses. Caspases, a family of cysteine proteases, are known to regulate cytokine maturation and apoptosis. Here, we show that caspase-11, a mammalian pro-inflammatory caspase, regulates cell migration
Critical roles of actin-interacting protein 1 in cytokinesis and chemotactic migration of mammalian cells.
Kato, A; Kurita, S; Hayashi, A; Kaji, N; Ohashi, K; Mizuno, K
The Biochemical Journal null

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