MABE229

Anti-phospho-Cdk1 (Thr14, Tyr15)Antibody, clone CP3.2

clone CP3.2, from mouse

• Sinônimo(s): Cyclin-dependent kinase 1, CDK1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: CP3.2, monoclonal
• reatividade de espécies: human
• técnica(s): immunocytochemistry: suitable; western blot: suitable
• Isotipo: IgG1κ
• nº de adesão NCBI: NP_001777
• nº de adesão UniProt: P06493
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: phosphorylation (pThr14, pTyr15)
• Informações sobre genes: human ... CDK1(983)

Descrição

Descrição geral

Cyclin dependent kinase 1 (Cdk1) regulates cell cycle progression. It is active as a heterodimeric complex with cyclins. Cdk1 associates with cyclin A at the end of S phase and targets proteins such as p53, BARD1, and BRCA2 involved in cell cycle progression, and others involved in DNA replication. At the end of the G2 phase, Cdk1 associates with cyclin B1 and B2 to phosphorylate multiple protein targets required for transition from G2 to M phase, and progression through M phase. Cdk1 itself undergoes phosphorylation at Thr14 and Tyr15 by PKMYT1, which prevents nuclear translocation of Cdk1. Cdk1 is also phosphorylated at Tyr15 by WEE1/2 which inhibits both Cdk1 activity and transition from G2 to M phase. Conversely, dephosphorylation of Thr14 and Tyr15 by CDC25A/B activates Cdk1 and triggers mitosis. Cdk1 may also regulate epigenetic gene silencing through phosphorylation of EZH2 and maintenance of histone H3 lysine 27 trimethylation, which has been implicated in cancer.

Especificidade

This antibody recognizes Cdk1 phosphorylated at Thr14 and phosphorylated at Tyr15.

Imunogênio

Epitope: Phosphorylated Thr14 and phosphorylated Tyr15

Linear peptide corresponding to human Cdk1 phosphorylated at Thr14 and phosphorylated at Tyr15.

Aplicação

Anti-phospho-Cdk1 (Thr14, Tyr15) Antibody, clone CP3.2 is a mouse monoclonal antibody for detection of phospho-Cdk1 (Thr14, Tyr15) also known as Cyclin-dependent kinase 1, or Cell division control protein 2 homolog & has been validated in WB, ICC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Qualidade

Evaluated by Western Blot in UV treated and untreated HeLa cell lysates.

Western Blot Analysis: A 1:1,000 dilution from a representative lot detected phospho-Cdk1 (Thr14, Tyr15) in UV treated HeLa cell lysates.

Descrição-alvo

~34 kDa observed. An uncharacterized band may be observed at ~62 kDa in some cell lysates.

forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
UV treated and untreated HeLa cell lysates

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable