Pular para o conteúdo
Merck
Todas as fotos(1)

Key Documents

MAB874

Sigma-Aldrich

Anti-Papillomavirus Antibody, 16, 18 E6 protein, clone C1P5

clone C1P5, Chemicon®, from mouse

Sinônimo(s):

HPV

Faça loginpara ver os preços organizacionais e de contrato


About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

C1P5, monoclonal

reatividade de espécies

human

fabricante/nome comercial

Chemicon®

técnica(s)

immunohistochemistry: suitable
western blot: suitable

Isotipo

IgG

Condições de expedição

wet ice

Descrição geral

Human papilloma viruses (HPVs) can be classified as either high risk or low risk according to their association with cancer. HPV16 and HPV18 are the most common of the high risk group while HPV6 and HPV11 are among the low risk types. Approximately 90% of cervical cancers contain HPV DNA of the high risk types. Mutational analysis have shown that the E6 and E7 genes of the high risk HPVs are necessary and sufficient for HPV transforming function. The specific interactions of the E6 and E7 proteins with p53 and pRB, respectively, correlate with HPV high and low risk classifications. The high risk HPV E7 proteins bind to pRB with a higher affinity than do the low risk HPV proteins, and only the high risk HPV E6 proteins form detectable complexes with p53 in vitro.

Especificidade

Recognizes Human papilloma virus type 18 early E6 protein. The antibody cross reacts with E6 protein from HPV type 16.

Imunogênio

C1P5 was made against HPV type 18 E6 protein produced in bacteria.
Epitope: 16, 18 E6 protein

Aplicação

Detect Papillomavirus using this Anti-Papillomavirus Antibody, 16, 18 E6 protein, clone C1P5 validated for use in IH & WB.
Immunoblotting: Reactive with a 16.5-18 kDa band in reduced westerns of overexpressing cells lines corresponding to HPV E6 protein in cells derived from human cervical carcinoma. {Banks, et al 1987}

Immunohistochemistry: Shows reactivity on formalin fixed tissue. Dilute with buffer pH 7.5-8.0 to desired working volumes. For extensive dilution, protein containing or other stabilizing medium should be used.

Immunopreciptiation: Immunoprecipiates 16.5 kDa band from human cervical carcinoma extracts {Banks, et al, 1987}.

Final working dilutions must be determined by end user.
Research Category
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral

Descrição-alvo

16.5-18 kDa

forma física

Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified

Armazenamento e estabilidade

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Nota de análise

Control
Human cervical carcinoma extract

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Não está encontrando o produto certo?  

Experimente o nosso Ferramenta de seleção de produtos.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Akiko Kobayashi et al.
The American journal of pathology, 160(1), 151-164 (2002-01-12)
The exact role of the mucosal immune response in the pathogenesis of human papillomavirus (HPV)-related premalignant and malignant diseases of the genital tract is poorly understood. We used immunohistochemical analysis to characterize immune cells in normal cervix (N = 21)
Ishita Gupta et al.
Pathogens (Basel, Switzerland), 9(6) (2020-06-12)
Colorectal cancer (CRC) is a common malignancy with a high mortality rate worldwide. It is a complex, multifactorial disease that is strongly impacted by both hereditary and environmental factors. The role of microbes (e.g., viruses) in the pathogenesis of CRC
Maurizio Chiriva-Internati et al.
European journal of immunology, 32(1), 30-38 (2001-12-26)
Adeno-associated virus (AAV) is able to efficiently deliver a cytokine gene into dendritic cells (DC). Improvements in T cell priming by DC might be effected by the delivery of antigen genes into DC, resulting in continuous protein expression, as most
L Banks et al.
The Journal of general virology, 68 ( Pt 5), 1351-1359 (1987-05-01)
We recently reported the expression of human papillomavirus type 18 (HPV-18) E6 protein in bacteria and the production of anti-E6 polyclonal antibodies. This work has now been extended with the production of a panel of monoclonal antibodies against the HPV-18
Martha Luevano et al.
Virology, 405(1), 31-40 (2010-06-18)
We have developed microarrays with all eight proteins encoded by 13 different human papillomavirus types associated with anogenital cancer (HPV-16, -18, -31, -33, -35, -45, and -53), genital warts (HPV-6 and -11), or skin lesions (HPV-1, -2, -4, and -5).

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica