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Merck
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Key Documents

MAB3776

Sigma-Aldrich

Anti-MDM2 Antibody, clone SMP14

clone SMP14, Chemicon®, from mouse

Sinônimo(s):

Murine Double Minute 2

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

SMP14, monoclonal

reatividade de espécies

rat, human, mouse

fabricante/nome comercial

Chemicon®

técnica(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable

Isotipo

IgG1

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... MDM2(4193)
mouse ... Mdm2(17246)
rat ... Mdm2(314856)

Descrição geral

MDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated (Zhang & Prives, 2001). SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.

Especificidade

Recognizes human MDM2.

Imunogênio

Synthetic peptide corresponding to residues 154-167 (CSRPSTSSRRRAISE) of human MDM2 cross-linked to KLH using glutaldehyde.

Aplicação

Research Sub Category
Ubiquitin & Ubiquitin Metabolism

RNA Binding Protein (RBP)
Use Anti-MDM2 Antibody, clone SMP14 (Mouse Monoclonal Antibody) validated in IP, IHC, IHC(P) to detect MDM2 also known as Murine Double Minute 2.
Western blot

Immunohistochemistry (Frozen and Paraffin-embedded sections)

Immunoprecipitation

ELISA

Optimal working dilutions must be determined by end user.

forma física

Format: Purified
Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide

Nota de análise

Control
POSITIVE CONTROL: Breast carcinoma

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Visite a Biblioteca de Documentos

Cyclin a-CDK phosphorylation regulates MDM2 protein interactions.
Zhang, T and Prives, C
The Journal of Biological Chemistry, 276, 29702-29710 (2001)
Richard O Adeyemi et al.
Journal of virology, 86(15), 8328-8332 (2012-05-25)
The DNA damage response to infection with minute virus of mice (MVM) leads to activated p53; however, p21 levels are reduced via a proteasome-mediated mechanism. This loss was sustained, as virus replicated in infected cells held at the G(2)/M border.
Gengze Wu et al.
Circulation, 130(17), 1452-1465 (2014-08-27)
Long noncoding RNAs (lncRNAs) have recently been implicated in many biological processes and diseases. Atherosclerosis is a major risk factor for cardiovascular disease. However, the functional role of lncRNAs in atherosclerosis is largely unknown. We identified lincRNA-p21 as a key
S M Picksley et al.
Oncogene, 9(9), 2523-2529 (1994-09-01)
The function of p53 is modulated by binding to a number of cellular and viral proteins, such as MDM2 and SV40 large T antigen. An initial immunochemical characterization of the p53-MDM2 complex in a rat fibroblast cell line (Clone 6)

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