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Documentos Principais

MAB3582

Sigma-Aldrich

Anti-Fascin Antibody, clone 55K2

clone 55K2, Chemicon®, from mouse

Sinônimo(s):

Anti-FAN1, Anti-SNL, Anti-p55

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified antibody

tipo de produto de anticorpo

primary antibodies

clone

55K2, monoclonal

reatividade de espécies

echinoderm, sea urchin, gerbil, human

reatividade da espécie (prevista por homologia)

mammals

fabricante/nome comercial

Chemicon®

técnica(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

Isotipo

IgG1

adequação

not suitable for immunoprecipitation

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... FSCN1(6624)

Especificidade

Fascin. The antibody is strongly positive when antigen-presenting dendritic cells are matured. MAB3582 is a sensitive marker for Reed-Sternberg Cells of Hodgkin′s Disease. The protein is an actin bundling protein specifically localized in filopodia and growth cones.

Imunogênio

Human fascin

Aplicação

Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-Fascin Antibody, clone 55K2 is validated for use in WB, IC, IH(P) for the detection of Fascin.
Western blot. Recognizes a protein of 55 kDa. Suggested working dilution is 1:100-1:1,000. Suggested blocking buffer is 3% BSA in PBS or 3% non fat milk in PBS.

Immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Suggested working dilution is 1:100-1:1,000.

Immunocytochemistry on cold methanol fixed cultured cells. Suggested working dilution is 1:100-1:1,000.

Optimal working dilutions must be determined by the end user.

forma física

Format: Purified
Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.

Armazenamento e estabilidade

Maintain at 2-8°C in undiluted aliquots up to 6 months after date of receipt.

Nota de análise

Control
POSITIVE CONTROL:

Hela cells, neuronal tissues, antigen presenting dendritic cells.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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S Al-Saad et al.
British journal of cancer, 99(9), 1476-1483 (2008-10-16)
Vimentin, nuclear factor-kappaB (NF-kappaB) p105, fascin, E-cadherin, TGF-beta, Par6 and atypical PKC are molecular markers that play an important role in cell differentiation. Herein, we investigate their prognostic impact in primary non-small-cell carcinoma (NSCLC). Tumour tissue samples from 335 resected
The prognostic impact of TGF-?1, fascin, NF-?B and PKC-? expression in soft tissue sarcomas.
Valkov, A; Sorbye, SW; Kilvaer, TK; Donnem, T; Smeland, E; Bremnes, RM; Busund, LT
Testing null
Protein expression dynamics during postnatal mouse brain development.
Laeremans, A; Van de Plas, B; Clerens, S; Van den Bergh, G; Arckens, L; Hu, TT
Journal of experimental neuroscience null
C S Cohan et al.
Cell motility and the cytoskeleton, 48(2), 109-120 (2001-02-13)
Growth cones at the distal tips of growing nerve axons contain bundles of actin filaments distributed throughout the lamellipodium and that project into filopodia. The regulation of actin bundling by specific actin binding proteins is likely to play an important
S Yamashiro-Matsumura et al.
The Journal of cell biology, 103(2), 631-640 (1986-08-01)
The 55-kD protein is a new actin-bundling protein purified from HeLa cells (Yamashiro-Matsumura, S., and F. Matsumura, 1985, J. Biol. Chem., 260:5087-5097). We have prepared monoclonal antibodies against the 55-kD protein and examined its intracellular localization, as well as its

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