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Key Documents

MAB3422

Sigma-Aldrich

Anti-Histone Antibody, clone H11-4

clone H11-4, Chemicon®, from mouse

Sinônimo(s):

Anti-Histone antibody

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified antibody

tipo de produto de anticorpo

primary antibodies

clone

H11-4, monoclonal

reatividade de espécies

hamster, bovine, opossum, rat, human, Xenopus

reatividade da espécie (prevista por homologia)

mammals

fabricante/nome comercial

Chemicon®

técnica(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

Isotipo

IgG1

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... H2AC21(317772)

Descrição geral

There are 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. The five major classes of histones are H1/H5, H2A, H2B, H3, and H4. Histones H2A, H2B, H3 and H4 are known as the core histones, while histones H1 and H5 are known as the linker histones. Histones feature a main globular domain and a long N terminal tail which are involved with the structure of the nucleosomes of the ′beads on a string′ structure. They are the chief protein components of chromatin, acting as spools around which DNA winds, and play a role in gene regulation.

Especificidade

Due to the highly conserved nature of histones, this antibody is predicted to have wide species reactivity.
The antibody binds specifically to an antigen determinant, which is present on all five histone proteins. The antibody recognizes the histones H1, H2A, H2B, H3 and H4 with comparable sensitivity.

Imunogênio

Purified nuclear fractions of HeLa cells

Aplicação

Detect Histone with Anti-Histone Antibody, clone H11-4 (Mouse Monoclonal Antibody), that has been shown to work in ICC, IHC & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Western Blot Analysis:
1:500 dilution of a previous lot was used.

Immunocytochemistry:
A 1-5 µg/mL concentration of a previous lot was used on Immunocytochemistry.

Immunohistochemistry:
A 1-5 μg/mL concentration of a previous lot was used on Immunohistochemistry.

Optimal working dilutions must be determined by end user.

Qualidade

Routinely evaluated by Western Blot on Jurkat lysates.

forma física

Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 in buffer containing 0.02M phosphate buffer, pH 7.6 with 0.25M NaCl and 0.1% sodium azide

Armazenamento e estabilidade

Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
The Iyophilized antibody is stable at 2–8°C. Store the reconstituted antibody in aliquots at -15 to -25°C for up to 12 months.Repeated freezing and thawing should be avoided.

Nota de análise

Control
All tissue

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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