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Key Documents

MAB339

Sigma-Aldrich

Anti-GABA A Receptor α1 chain Antibody, NT, clone BD24

clone BD24, Chemicon®, from mouse

Sinônimo(s):

Anti-DEE19, Anti-ECA4, Anti-EIEE19, Anti-EJM, Anti-EJM5

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

BD24, monoclonal

reatividade de espécies

human, pig, avian, bovine

não deve reagir com

feline, rat

fabricante/nome comercial

Chemicon®

técnica(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG1

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... GABRA1(2554)

Especificidade

Reacts with the alpha 1-chain of the GABAA receptor in bovine and human but not rat

Imunogênio

Epitope: N-terminus
Purified GABA/benzodiazepine receptor from bovine cortex.

Aplicação

Anti-GABA A Receptor α1 chain Antibody, N-terminus, clone BD24 is an antibody against GABA A Receptor α1 chain for use in IH, IP & WB.
Immunohistochemistry: 10-20 μg/mL * See protocol below.

Western blot: 20 μg/mL

Immunoprecipitation

Optimal working dilutions must be determined by end user.

APPLICATION NOTES FOR MAB339

IMMUNOHISTOCHEMISTRY

1) Fresh tissue (human) should be used (3-6 hours postmortem). The tissue should be immersion fixed in 2% paraformaldehyde/0.1% glutaraldehyde. Prepare 50 mm sections and store in cryoprotective solution at -15°C.

2) Sections should be incubated floating in suitable small vials.

3) Block endogenous peroxidase with 100 mmol/L Tris-HCl, 150 mmol/L NaCl, pH 7.4 (Buffer A) containing 3% H2O2 (v/v) and 10% methanol for 20 min at room temperature.

4) Wash sections in Buffer A. Incubate sections in Buffer A containing 5% fetal bovine serum (v/v) and 0.1-0.5% Triton X-100 (v/v).

5) Wash sections in Buffer A. Incubate sections with MAB339 (diluted 10-20 mg/mL in Buffer A containing 5% fetal bovine serum (v/v) and 0.1-0.5% Triton X-100 (v/v) for 12-36 hours at +4°C

6) Wash sections in Buffer A.

7) Detect with standard secondary antibody detection system (PAP, ABC, etc.).

8) Wash sections in Buffer A.

9) Mount sections on chrome alum-coated slides, dry, dehydrate, and apply coverslips.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

forma física

Format: Purified
Liquid. Buffer = 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

Armazenamento e estabilidade

Maintain lyophilized material at +2-8°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Visite a Biblioteca de Documentos

Lisa L Gomez et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 22(16), 7027-7044 (2002-08-15)
At the postsynaptic membrane of glutamatergic synapses, the cAMP-dependent protein kinase (PKA), protein kinase C (PKC), and calcineurin (CaN) anchoring protein AKAP79/150 is recruited to NMDA and AMPA glutamate receptors by postsynaptic density (PSD)-95 family membrane-associated guanylate kinase (MAGUK) scaffold
Novel ?1 and ?2 GABAA receptor subunit mutations in families with idiopathic generalized epilepsy.
Pamela Lachance-Touchette,Patricia Brown,Caroline Meloche,Peter Kinirons,Line Lapointe et al.
The European Journal of Neuroscience null
Differential localization of GABAA receptor subunits within the substantia nigra of the human brain: an immunohistochemical study.
H J Waldvogel,K Baer,W-P Gai,R T Gilbert,M I Rees,H Mohler,R L M Faull
The Journal of Comparative Neurology null
Ivan Milenkovic et al.
Brain structure & function, 223(3), 1501-1518 (2017-11-24)
The function, regulation and cellular distribution of GABAA receptor subunits have been extensively documented in the adult rodent brain and are linked to numerous neurological disorders. However, there is a surprising lack of knowledge on the cellular (sub-) distribution of
Cell type-specific effect of hypoxia and platelet-derived growth factor-BB on extracellular matrix turnover and its consequences for lung remodeling.
Karakiulakis, G; Papakonstantinou, E; Aletras, AJ; Tamm, M; Roth, M
The Journal of Biological Chemistry null

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