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Merck
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Key Documents

MAB316

Sigma-Aldrich

Anti-GABA Antibody, clone 5A9

clone 5A9, Chemicon®, from mouse

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified antibody

tipo de produto de anticorpo

primary antibodies

clone

5A9, monoclonal

reatividade da espécie (prevista por homologia)

mammals

fabricante/nome comercial

Chemicon®

técnica(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

Isotipo

IgG1

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... GABRA1(2554)

Especificidade

Specific for GABA and does not cross-react with other amino acids.

Imunogênio

GABA-coupled to BSA with glutaraldehyde.

Aplicação

Anti-GABA Antibody, clone 5A9 detects level of GABA & has been published & validated for use in ELISA, IH & WB.
Immunohistochemistry on frozen sections if perfusion fixed. Glutaraldehyde must be included in fixative with paraffin sections: 1:200-1:400. Typical fixative might be 4% PFA, 1% glutaraldehyde in 0.1M sodium phosphate buffer, pH 7.3 .

Immunoblotting not recommended due to low molecular wgt: 103Da.

Optimal working dilution must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

forma física

Format: Purified
Protein A purified
Purified mouse monoclonal in PBS, pH 7.4, 1% BSA, and 0.1% sodium azide as a preservative.

Armazenamento e estabilidade

Maintain refrigerated at 2–8°C for up to 6 months.

Nota de análise

Control
Myeloblasts, lymphocytes, erythroblasts

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Alain C Burette et al.
The Journal of comparative neurology, 522(9), 2164-2178 (2014-03-19)
The 53 kDa insulin receptor substrate protein (IRSp53) is highly enriched in the brain. Despite evidence that links mutations of IRSp53 with autism and other neuropsychiatric problems, the functional significance of this protein remains unclear. We used light and electron
GABAergic Interneurons are the Major Postsynaptic Targets of Median Raphe Afferents in the Rat Dentate Gyrus.
Halasy, K., et al.
The European Journal of Neuroscience, 4, 144-153 (1992)
E Szabat et al.
Neuroscience, 47(2), 409-420 (1992-01-01)
A monoclonal antibody, 115AD5, was raised against GABA coupled to bovine serum albumin. The monoclonal antibody 115AD5 also reacted with other GABA-protein conjugates. The specificity of the monoclonal antibody was corroborated by enzyme-linked immunoassay, dot-immunobinding experiments and immunostaining of rat
Identification of major S-nitrosylated proteins in murine experimental autoimmune encephalomyelitis.
Oscar A Bizzozero,Jianzheng Zheng
Journal of Neuroscience Research null
S Westerholz et al.
Neuroscience, 168(2), 573-589 (2010-03-27)
Early in development spontaneous activity modulates survival and connectivity of neurons and thus plays a crucial role in the formation of neural networks. The emergence of synchronous activity in cultured neocortical networks initially is driven by large GABAergic interneurons. Here

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