MAB1012
Anti-Alkaline Phosphatase Antibody, E. coli, bacterial only
ascites fluid, Chemicon®
Sinônimo(s):
AP, Alk. Phos.
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
ascites fluid
tipo de produto de anticorpo
primary antibodies
clone
monoclonal
reatividade de espécies
E. coli
fabricante/nome comercial
Chemicon®
técnica(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG2a
nº de adesão UniProt
Condições de expedição
dry ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
Escherichia coli ... PhoA(945041)
Especificidade
Alkaline phosphatase (AP). MAB1012 has a high affinity and recognizes an AP determinant resistant to denaturation by SDS-PAGE. It is therefore ideally suited for sensitive and specific detection of AP fusion proteins by Western blot analysis of E. coli transformants expressing fusion products.
Imunogênio
Epitope: E. coli, bacterial only
F201C1B
Aplicação
Anti-Alkaline Phosphatase Antibody, E. coli, bacterial only detects level of Alkaline Phosphatase & has been published & validated for use in IP, WB & IC.
Western blot at 1:5,000. 42-45kDa on SDS-PAGE,reducing gels for natural E. coli alkaline phosphatase monomer. Alkaline phosphatase fusion proteins will vary depending upon the target fusion protein.
Immunocytochemistry: reacts with E.coli. AP fusion protein targets in acetone fixed cell preparations. 1:4000, other fixatives or conditions untested.
ASSAY:
Preparation of E. coli TnphoA transformants: E. coli strain CC118 was transformed with plasmid pGEM-3Z containing TnphoA insertional mutations in the p101 gene of Mycoplasma hyorhinis, which encodes a protein with a typical N-terminal prokaryotic single peptide (Yogev et al. 1991).
Identification of fusion protein with MAB1012 transformants: Transformants are grown in 2XYT medium to OD600=0.6. Cells were centrifuged 3 minutes at 10,000 x g, suspended in SDS-PAGE sample buffer, heated at 100°C for 5 minutes, frozen and thawed and centrifuged as above at room temperature to remove insoluble material. The sample is applied at 9% to a SDS-PAGE gel, and Western immunoblot is performed as described (Yogev et al. 1991).
Immunoprecipitation: 5μL of antibody per 500μL of lysate in RIPA or 0.5% triton X-100 solutions.
Optimal working dilutions must be determined by end user.
Immunocytochemistry: reacts with E.coli. AP fusion protein targets in acetone fixed cell preparations. 1:4000, other fixatives or conditions untested.
ASSAY:
Preparation of E. coli TnphoA transformants: E. coli strain CC118 was transformed with plasmid pGEM-3Z containing TnphoA insertional mutations in the p101 gene of Mycoplasma hyorhinis, which encodes a protein with a typical N-terminal prokaryotic single peptide (Yogev et al. 1991).
Identification of fusion protein with MAB1012 transformants: Transformants are grown in 2XYT medium to OD600=0.6. Cells were centrifuged 3 minutes at 10,000 x g, suspended in SDS-PAGE sample buffer, heated at 100°C for 5 minutes, frozen and thawed and centrifuged as above at room temperature to remove insoluble material. The sample is applied at 9% to a SDS-PAGE gel, and Western immunoblot is performed as described (Yogev et al. 1991).
Immunoprecipitation: 5μL of antibody per 500μL of lysate in RIPA or 0.5% triton X-100 solutions.
Optimal working dilutions must be determined by end user.
forma física
Ascites. Contains no preservative.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Sequence and TnphoA analysis of a Mycoplasma hyorhinis protein with membrane export function.
Yogev, D, et al.
Journal of Bacteriology, 173, 2035-2044 (1991)
A genetic approach to analyzing membrane protein topology.
Manoil, C and Beckwith, J
Science (New York, N.Y.), 233, 1403-1408 (1986)
Both the stroma and thylakoid lumen of tobacco chloroplasts are competent for the formation of disulphide bonds in recombinant proteins.
Julia Bally,Eric Paget,Michel Droux,Claudette Job,Dominique Job,Manuel Dubald
Plant Biotechnology Journal null
Fusions of secreted proteins to alkaline phosphatase: an approach for studying protein secretion.
Hoffman, C S and Wright, A
Proceedings of the National Academy of Sciences of the USA, 82, 5107-5111 (1985)
TnphoA: a transposon probe for protein export signals.
Manoil, C and Beckwith, J
Proceedings of the National Academy of Sciences of the USA, 82, 8129-8133 (1985)
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