IP05
Protein G Plus/Protein A Agarose Suspension
Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.
Sinônimo(s):
Affinity resin, Protein G/Agarose
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About This Item
Código UNSPSC:
41116133
NACRES:
NA.56
Produtos recomendados
Formulário
slurry (Liquid)
contém
≤0.1% sodium azide as preservative
fabricante/nome comercial
Calbiochem®
condição de armazenamento
do not freeze
técnica(s)
protein purification: suitable
adequação
suitable for microbiology
Condições de expedição
wet ice
temperatura de armazenamento
2-8°C
Descrição geral
Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification.
Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.
Aplicação
Immunoprecipitation (see comments)
Advertência
Toxicity: Standard Handling (A)
forma física
33% slurry in PBS.
Outras notas
Agarose solution is supplied ready to use. Protein G Plus/Protein A Agarose immunoprecipitation reagent is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl of solution per µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
Informações legais
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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