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Key Documents

GF155

Sigma-Aldrich

Epidermal Growth Factor Protein, Recombinant mouse

The Epidermal Growth Factor (EGF) is a polypeptide growth factor, which stimulates the proliferation of a wide range of epidermal & epithelial cells.

Sinônimo(s):

EGF

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About This Item

Código UNSPSC:
12352202
eCl@ss:
32160405
NACRES:
NA.77

Nível de qualidade

Ensaio

>98% (SDS-PAGE)

atividade específica

≥6.6 x 106 U/mg

fabricante/nome comercial

Chemicon®

Impurezas

<0.1 ng/μg Endotoxin (of EGF; 1EU/ug)

entrada

sample type epithelial cells
sample type mesenchymal stem cell(s)
sample type neural stem cell(s)

nº de adesão UniProt

Condições de expedição

dry ice

Descrição geral

Epidermal Growth Factor (EGF) is a polypeptide growth factor, which stimulates the proliferation of a wide range of epidermal and epithelial cells. Recombinant murine EGF is a 6.2 kDa protein containing 54 amino acid residues.
Product Source: E.coli

Aplicação

For most in vitro applications, EGF exerts its biological activity in the concentration range of 0.5 to 25.0 ng/mL. Responding cells are (partial list): Epidermal mesothelial cells.
The Epidermal Growth Factor (EGF) is a polypeptide growth factor, which stimulates the proliferation of a wide range of epidermal & epithelial cells.

Ligação

Replaces: GF123

forma física

Lyophilized from sterile filtered 10 mM Sodium Phosphate, pH 7.5, containing no additives.

Armazenamento e estabilidade

The lyophilized EGF, though stable at room temperature, is best stored desiccated at -20°C. Reconstitute with water to a concentration of 0.1 - 1 mg/mL. This solution can be diluted into other buffered solutions and stored at 4°C for 1 week or -20°C for future use. Reconstituted EGF should be maintained at -20°C in undiluted aliquots for up to one year.

Nota de análise

Specific Activity: Mouse EGF is fully biologically active when compared to standards.The ED50, as determined by its dose-dependent mitogenic activity on BALB/c 3T3 cells, is ≤ 0.15 ng/mL

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Min-Yi Ou et al.
Development (Cambridge, England), 147(10) (2020-04-24)
Cortex development is controlled by temporal patterning of neural progenitor (NP) competence with sequential generation of deep and superficial layer neurons, but underlying mechanisms remain elusive. Here, we report a role for heterogeneous nuclear ribonucleoprotein A3 (HNRNPA3) in regulating the

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