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Merck
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Key Documents

CP70

Sigma-Aldrich

Anti-Fibronectin (Ab-3) Mouse mAb (FBN11)

liquid, clone FBN11, Calbiochem®

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.43

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified antibody

tipo de produto de anticorpo

primary antibodies

clone

FBN11, monoclonal

forma

liquid

contém

≤0.1% sodium azide as preservative

reatividade de espécies

rat, human, mouse

fabricante/nome comercial

Calbiochem®

condição de armazenamento

do not freeze

Isotipo

IgG1

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... FN1(2335)
mouse ... Fn1(14268)
rat ... Fn1(25661)

Descrição geral

Anti-Fibronectin (Ab-3), mouse monoclonal, clone FBN11, recognizes dimeric fibronectin in SWI56 cells and normal kidney. It is validated for use in WB, IF, IP & IHC (frozen and paraffin sections).
Protein G purified mouse monoclonal antibody. Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin.
Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin in SWI56 cells and normal kidney tissue.

Imunogênio

recombinant human fibronectin

Aplicação

Frozen Sections (0.5-1 µg/ml)

Immunoblotting (0.5-1 µg/ml)

Immunofluorescence (see comments)

Immunoprecipitation (2 µg/mg protein lysate)

Paraffin Sections (0.5-1 µg/ml, heat pre-treatment required)

Embalagem

Please refer to vial label for lot-specific concentration.

Advertência

Toxicity: Standard Handling (A)

Nota de análise

Positive Control
SW 156 cells or normal kidney tissue

Outras notas

Clark, R.A. 2001. Ann. N.Y. Acad. Sci.936, 355.
Armstrong, P.B. and Armstrong, M.T. 2000. Biochim. Biophys. Acta1470, O9.
Barilla, M.L. and Carsons, S.E. 2000. Semin. Arthritis Rheum.29, 252.
Yamada, K.M. 1989. In Fibronectin, Mosher, D.F., ed., p. 47.
Rouslahti, E. and Pierschbacher, M.D. 1986. Cell44, 517.
Pytela, R., et al. 1985. Cell40, 191.
Exhibits staining of the connective tissue matrix when used in immunohistochemistry. This antibody is suitable for both reduced and non-reduced protein by immunoblotting. For staining of formalin-fixed tissues, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. This antibody has also been reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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