AC113

Anti-Neomycin Phosphotransferase II Antibody | AC113

from rabbit, purified by affinity chromatography

• Sinônimo(s): Aminoglycoside 3′-phosphotransferase, APH(3′)-II, APH(3′)II, Kanamycin kinase, type II, Neomycin-kanamycin phosphotransferase type II
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: human
• técnica(s): western blot: suitable
• nº de adesão NCBI: YP_788126
• nº de adesão UniProt: P00552
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified

Descrição

Descrição geral

Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin^®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.

Especificidade

This antibody reacts to lysates from cells transfected or transformed with a plasmid containing a kanamycin/neomycin resistance gene, nptII (Aminoglycoside 3′-phosphotransferase).

Imunogênio

Recombinant protein corresponding to full length neomycin phosphotransferase (NPT) II.

Aplicação

Research Category
Secondary & Control Antibodies

Research Sub Category
Kinases & Phosphatases

Qualidade

Evaluated by Western Blot in untreated and neomycin treated HEK293 cells transfected with pUSEamp(-).

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Neomycin phosphotransferase II in 10 µg of neomycin treated HEK293 cells transfected with pUSEamp(-).

Descrição-alvo

~29 kDa observed.

forma física

Affinity purified

Hek 293 +/- transfection with pUSEamp(-)and neomycin treatment.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
Untreated and neomycin treated HEK293 cells transfected with pUSEamp(-).

Informações legais

Geneticin is a registered trademark of Gibco BRL Life Technologies, Inc.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°C): Not applicable