ABT80

Anti-Girdin, coiled-coil region Antibody

from rabbit, purified by affinity chromatography

• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: monkey, human
• reatividade da espécie (prevista por homologia): rat
• técnica(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• nº de adesão NCBI: XP_223709
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... CCDC88A(55704)

Descrição

Descrição geral

Girdin (Ccdc88a; APE, GIV, or HkRP1) is a ubiquitous protein belonging to the CCDC88 family. Girdin is an actin-binding protein which interacts with actin via its C-terminal domain, and may play a crucial role in cell motility and cytoskeleton remodeling. These effects may be regulated by a reciprocal relationship between Girdin and Akt. Akt phosphorylates Girdin, and Girdin binds to Akt and enhances phosphorylation of Akt, thereby promoting phosphorylation of Akt’s downstream targets such as GSK-3β and FOXO1A. Girdin/Akt signaling may play an important role in cancer by facilitating the proliferation of cancer cells.

Especificidade

Other Homologies: Mouse (97% sequence homology).

Imunogênio

GST-tagged recombinant protein corresponding to the coiled coil domain of rat Girdin.

Aplicação

Immunocytochemistry Analysis: A 1:1000 dilution from a representative lot detected Girdin in Cos-7 cells (Prof. P. Ghosh, Department of Medicine, Division of Gastroenterology and Hepatology, UCSD School of Medicine and Veterans Affairs Medical Center).

Western Blot Analysis: A representative lot detected Girdin in Cos-7 cell lysate. Uncharacterized bands may be observed in some cell lysates.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Girdin in HeLa cell lysates (Prof. P. Ghosh, Department of Medicine, Division of Gastroenterology and Hepatology, UCSD School of Medicine and Veterans Affairs Medical Center). Uncharacterized bands may be observed in some cell lysates.

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

This Anti-Girdin, coiled-coil region Antibody is validated for use in ICC, Western Blotting, Immunoprecipitation for the detection of Girdin, coiled-coil region.

Qualidade

Immunocytochemistry Analysis: A 1:1000 dilution of this antibody detected Girdin in HeLa cells (Prof. A. Ghosh, Division of Biology, UCSD).

Descrição-alvo

~250 kDa observed. Some uncharacterized bands may be observed in some cell lysates.

forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
HeLa cells

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable