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Merck
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Key Documents

ABS994

Sigma-Aldrich

Anti-phospho-Glucocorticoid receptor Antibody, Ser226

from rabbit, purified by affinity chromatography

Sinônimo(s):

Nuclear receptor subfamily 3 group C member 1, glucocorticoid receptor, GR

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

purificado por

affinity chromatography

reatividade de espécies

human

reatividade da espécie (prevista por homologia)

mouse (based on 100% sequence homology), rat (based on 100% sequence homology)

técnica(s)

western blot: suitable

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

phosphorylation (pSer226)

Informações sobre genes

human ... NR3C1(2908)

Descrição geral

Glucocorticoid receptor (GR), also known as Nuclear receptor subfamily 3 group C member 1, and encoded by the gene name NR3C1/ GRL, is receptor for glucocorticoids (GC). GR has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. GR affects inflammatory responses, cellular proliferation and differentiation in target tissues. Recent studies have shown that GR may act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. In addition, GR is involved in chromatin remodeling, and may play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic genes expression. Mutation in GR has been associated with Glucocorticoid resistance (GCRES): a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant. GR is widely expressed. In the heart, GR is detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node, as well as whole adult and fetal heart.

Imunogênio

Epitope: A region within the modulating domain
KLH-conjugated linear peptide corresponding to the domain of: Modulating region of Human Glucocorticoid receptor.

Aplicação

Research Category
Signaling
Research Sub Category
Lipid Metabolism & Weight Regulation
This Anti-phospho-Glucocorticoid receptor antibody is validated for use in Western Blotting for the detection of phospho-Glucocorticoid receptor.
Western Blotting Analysis: A representative lot of this antibody at 0.5 µg/mL dilution detected phosphorylated glucocorticoid receptor protein in transfected U2OS cell lysate (Chen, W., et al. Mol Endocrinol, August 2008, 22(8):1754–1766)
Western Blotting Analysis: A representative lot of this antibody at 0.5 µg/mL dilution detected phosphorylated glucocorticoid receptor protein in rat hepatoma cell lysate (Blind,R., et al. Journal of Steroid Biochemistry & Molecular Biology 109 (2008) 150–157)

Qualidade

Evaluated by Western Blotting on transfected HEK293 cell lysate

Western Blotting Analysis: 1 µg/mL dilution of this antibody detected phosphorylated glucocorticoid receptor in 6 µg of transfected HEK293 cell lysate

Descrição-alvo

~85 kDa observed

forma física

Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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Glucocorticoid receptor phosphorylation differentially affects target gene expression.
Chen, W; Dang, T; Blind, RD; Wang, Z; Cavasotto, CN; Hittelman, AB; Rogatsky, I; Logan et al.
Molecular Endocrinology null
Differential recruitment of glucocorticoid receptor phospho-isoforms to glucocorticoid-induced genes.
Blind, RD; Garabedian, MJ
The Journal of Steroid Biochemistry and Molecular Biology null

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