Pular para o conteúdo
Merck
Todas as fotos(1)

Key Documents

ABS524

Sigma-Aldrich

Anti-Lipin-2 Antibody

from rabbit, purified by affinity chromatography

Sinônimo(s):

Phosphatidate phosphatase LPIN2, Lipin-2

Faça loginpara ver os preços organizacionais e de contrato


About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

purificado por

affinity chromatography

reatividade de espécies

mouse

técnica(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

mouse ... Lpin2(64898)

Categorias relacionadas

Descrição geral

Lpin2, also known as Phosphatidate phosphatase LPIN2, and encoded by the gene Lpin2, plays important roles in controlling the metabolism of fatty acids. Lpin2 acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Lpin2 also acts as a nuclear transcriptional coactivator for PGC-1α protein. Lpin2 is localized to the cytoplasm and endoplasmic reticulum membrane but is also found in the nucleus. Lpin2 is expressed in many cells and tissues including liver, lung, kidney, placenta, prostate, and intestine. Expression is also seen in RBCs and at sites of lymphopoiesis in bone and spleen. Homozygous mutations in LPIN2 result in Majeed syndrome. Majeed syndrome is an autosomal recessive, autoinflammatory disorder characterized by chronic recurrent multifocal osteomyelitis and congenital dyserythropoietic anemia.

Imunogênio

Linear peptide corresponding to mouse Lipin-2.

Aplicação

Anti-Lipin-2 Antibody is an antibody against Lipin-2 for use in western blotting, IHC & IP.
Research Category
Signaling
Research Sub Category
Lipid Metabolism & Weight Regulation
Western Blotting Analysis: A representative lot detected Lipin-2 in mouse WAT cell lysate (Gropler, M. C., et al. (2008). JBC. 284(11):6763-6772).
Immunocytochemistry Analysis: A representative lot detected Lipin-2 in mouse cerebellum tissue (Mayurranjan, S., et al. (2013). PNAS. 110(2):642-647).

Qualidade

Evaluated by Western Blotting in mouse hepatocytes cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Lipin-2 in 10 µg of mouse hepatocytes cell lysate.

Descrição-alvo

~ 110 kDa observed

forma física

Affinity purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide with 50% glycerol.

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Não está encontrando o produto certo?  

Experimente o nosso Ferramenta de seleção de produtos.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Matthew C Gropler et al.
The Journal of biological chemistry, 284(11), 6763-6772 (2009-01-13)
Lipin 1 is a bifunctional intracellular protein that regulates fatty acid metabolism in the nucleus via interactions with DNA-bound transcription factors and at the endoplasmic reticulum as a phosphatidic acid phosphohydrolase enzyme (PAP-1) to catalyze the penultimate step in triglyceride
Jingquan He et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 31(7), 2893-2904 (2017-03-30)
Cancer cells reprogram their metabolism to increase the synthesis of macromolecules for rapid proliferation. Compared to fatty acids, much less is known about the synthesis of phospholipids, which is essential for membrane biogenesis in cancer cells. We found that LPIN1

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica