ABS2231

Anti-phospho RyR2 (Ser2808)

• Sinônimo(s): RYR-2, RyR2, hRYR-2, Cardiac muscle ryanodine receptor, Cardiac muscle ryanodine receptor-calcium release channel, Type 2 ryanodine receptor
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 200
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• peso molecular: calculated mol wt 564.57 kDa; observed mol wt ~565 kDa
• purificado por: affinity chromatography
• reatividade de espécies: rabbit
• reatividade da espécie (prevista por homologia): mouse, human
• embalagem: antibody small pack of 100 μg
• técnica(s): western blot: suitable
• Isotipo: IgG
• sequência de epítopo: C-terminal cytoplasmic domain
• nº de adesão de ID de proteína: NP_001026
• nº de adesão UniProt: Q92736
• temperatura de armazenamento: -10 to -25°C
• modificação pós-traducional do alvo: phosphorylation (pSer2808)
• Informações sobre genes: human ... RYR2(55193)

Descrição

Descrição geral

Ryanodine receptor 2 (UniProt: Q92736; also known as RYR-2, RyR2, hRYR-2, Cardiac muscle ryanodine receptor, Cardiac muscle ryanodine receptor-calcium release channel, Type 2 ryanodine receptor) is encoded by the RYR2 gene (Gene ID: 6262) in human. Ryanodine receptors are large ion channels that are located in the sarcoplasmic/endoplasmic reticulum membrane and are responsible for the release of Ca2+. They form homotetramers with a mushroom-like shape, consisting of a large cytoplasmic head and transmembrane stalk. In mammals three isoforms of RYR have been described (RYR 1-3) that are regulated by phosphorylation, redox modifications, and a variety of small proteins, and ions. Ryanodine receptor 2 is a multi-pass membrane protein with two cytoplasmic domains (aa 1-4281 and 4871-4967), six transmembrane domains, and one pore-forming domain (aa 4820-4829). It is the predominant form in cardiac muscle and is also expressed in Purkinje cells of cerebellum and cerebral cortex. It plays a key role in triggering cardiac muscle contraction and aberrant channel activation can lead to cardiac arrhythmia. Channel activity is modulated by ryanodine that binds to the open Ca2+-release channel with high affinity. At low concentrations, ryanodine maintains the channel in an open conformation, but high concentrations of ryanodine inhibit channel activity. Channel activity is also modulated by phosphorylation. Phosphorylation at serine 2808 and serine 2814 is shown to increase the open probability of the calcium channel. Phosphorylation is increased in failing heart, leading to calcium leaks, and increased cytoplasmic Ca2+ levels. (Ref.: Xiao, Z., et al. (2016). J. Biol. Chem. 291(47); 24528-24537; Lanner, JT., et al. (2010). Cold Spring Harb. Perspect. Biol. 2(11); a003996).

Especificidade

This rabbit polyclonal antibody detects Ryanodine receptor 2 (RyR2) phosphorylated on serine 2808.

Imunogênio

A KLH-conjugated linear peptide corresponding to 8 amino acids surrounding phosphoserine 2808 from human Ryanodine receptor 2.

Aplicação

Quality Control Testing

Evaluated by Western Blotting in rabbit skeleton muscle microsomal preparation.

Western Blotting Analysis (WB): A 1:300 dilution of this antibody detected RyR2 phosphorylated on serine 2808 in rabbit skeleton muscle microsomal preparations, but not in microsomes treated with protein phosphatase.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

forma física

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without azide

Reconstituição

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Armazenamento e estabilidade

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable