ABN1475

Anti-Serotonin receptor 3A/HTR3A

from rabbit, purified by affinity chromatography

• Sinônimo(s): 5-hydroxytryptamine receptor 3A, 5-HT-3, 5-HT3-A, 5-HT3A, 5-HT3R, 5-hydroxytryptamine receptor 3, Serotonin receptor 3A, Serotonin-gated ion channel receptor
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: human
• técnica(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• nº de adesão NCBI: NP_000860
• nº de adesão UniProt: P46098
• Condições de expedição: ambient
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... HTR3A(3359)

Descrição

Descrição geral

5-hydroxytryptamine receptor 3A (UniProt P46098; also known as 5-HT-3, 5-HT3-A, 5-HT3A, 5-HT3R, 5-hydroxytryptamine receptor 3, Serotonin receptor 3A, Serotonin-gated ion channel receptor) is encoded by the HTR3A (also known as 5HT3R, HTR3) gene (Gene ID 3359) in human. Hydroxytryptamine (5-HT) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT. 5-hydroxytryptamine receptor 3 (5-HT3R) is a ligand-gated ion channel involved in many neurophysiological functions and is widely expressed throughout the brain and in peripheral organs. Five different subunits (A through E) have been identified, with the homopentameric 5-HT3A being the most studied form. 5-HT3A is produced with a signal peptide sequence (a.a. 1-23), which is removed posttranslationally to yield the mature 4-transmembrane (a.a. 242-268, 274-292, 303-321, 456-475) ion channel with both its N- and C-terminal ends exposed extracellularly (a.a. 24-241 and 476-478).

Especificidade

This rabbit polyclonal antibody detects human Serotonin receptor 3A/HTR3A by targeting a sequence within the second cytoplasmic domain.

Imunogênio

KLH-conjugated linear peptide corresponding to a sequence from the second cytomplasmic domain of human Serotonin receptor 3A/HTR3A.

Aplicação

Anti-Serotonin receptor 3A/HTR3A Antibody, Cat. No. ABN1475, is a highly specific rabbit polyclonal antibody that targets HTR3A and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected serotonin receptor 3A/HTR3A in human prostate cancer, as well as in normal cerebral cortex, heart and tonsil tissue sections.

Research Category
Neuroscience

Qualidade

Evaluated by Western Blotting in human cerebral cortex tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected serotonin receptor 3A/HTR3A in 10 µg of human cerebral cortex tissue lysate.

Descrição-alvo

~55 kDa observed. 52,79/55.28 kDa (mature/pro-isoform 1; 5-HT3R-AS), 55.90/58.39 kDa (mature/pro-isoform 2; 5-HT3R-AL), 52.79/53.76 kDa (mature/pro-isoform 3), 52.79/55.89 kDa (mature/pro-isoform 4), 55.90/59.00 kDa (mature/pro-isoform 5). Uncharacterized bands may be observed in some lysate(s).

forma física

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable