ABE1863

Anti-PAGR1 (PA1)

from rabbit

• Sinônimo(s): PAXIP1-associated glutamate-rich protein 1, Glutamate-rich co-activator interacting with SRC11, GAS1, PAXIP1-associated protein 1, PTIP-associated protein 1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• reatividade de espécies: mouse, human
• embalagem: antibody small pack of 25 μg
• técnica(s): ChIP: suitable; immunocytochemistry: suitable; western blot: suitable
• Isotipo: IgG
• nº de adesão NCBI: NP_078792
• nº de adesão UniProt: Q9BTK6
• Condições de expedição: ambient
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... PAGR1(79447)

Descrição

Descrição geral

PAXIP1-associated glutamate-rich protein 1 (UniProt: Q9BTK6; also known as Glutamate-rich co-activator interacting with SRC1, GAS, PAXIP1-associated protein 1, PTIP-associated protein 1) is encoded by the PAGR1 (also known as C16orf53, PA1) gene (Gene ID: 79447) in human. It associates with the histone methyltransferase MLL3/4 complex, which suggests that it plays a role in epigenetic and transcriptional activation. However, in association with PAXIP1/PTIP is proposed to function at least in part independently of the MLL3/4 complex. It is proposed to be recruited by PAXIP1 to sites of DNA damage where the PAGR1:PAXIP1 complex is required for cell survival in response to DNA damage independently of the MLL3/4 complex. During immunoglobulin class switching in activated B-cells, it is involved in transcription regulation of downstream switch regions at the immunoglobulin heavy-chain (Igh) locus independently of the MLL3/4 complex. PAGR1 protein is reported to act as a transcriptional cofactor for estrogen receptor. It interacts with GR (NR3C1) and inhibits the GR transactivation.

Especificidade

This polyclonal antibody detects PAXIP1-associated glutamate-rich protein 1 in human and mouse.

Imunogênio

His-tagged full-length human recombinant PAXIP1-associated protein 1.

Aplicação

Anti-PAGR1 (PA1), Cat. No. ABE1863, is a highly specific rabbit polyclonal antibody that targets PAXIP1-associated glutamate-rich protein 1 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, and Western Blotting.

Western Blotting Analysis: A representative lot detected PAGR1 (PA1) in Western Blotting applications (Lee, J.E., et. al. (2013). Elife. 2:e01503; Gong, Z., et. al. (2009). J Biol Chem. 284(11):7284-93).

Chromatin Immunoprecipitation Analysis: A representative lot detected PAGR1 (PA1) in MEF′s (Cho, Y.W., et. al. (2009). Cell Metab. 10(1):27-39).

Immunocytochemistry Analysis: A representative lot detected PAGR1 (PA1) in U2OS & HeLa cells (Cho, Y.W., et. al. (2009). Cell Metab. 10(1):27-39).

Qualidade

Evaluated by Western Blotting in HEK293 cell nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PAGR1 (PA1) in 10 µg of HEK293 cell nuclear extract.

Descrição-alvo

~42 kDa observed; 27.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

forma física

Format: Purified

Outras notas

Concentration: Please refer to lot specific datasheet.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable