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Documentos Principais

AB5467

Sigma-Aldrich

Anti-AANAT Antibody

serum, Chemicon®

Sinônimo(s):

Arylalkylamine N-acetyltransferase, SNAT, Serotonin Acetylase, EC 2.3.1.87

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

forma do anticorpo

serum

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

reatividade de espécies

rat, sheep, fish, human, bovine

fabricante/nome comercial

Chemicon®

técnica(s)

western blot: suitable

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

dry ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... AANAT(15)

Especificidade

Recognizes AANAT (Arylalkylamine N-acetyltransferase). The antibody specifically recognizes the phosphorylated N-terminal PKA site of AANAT (pT31) (Ganguly et al. 2005). The antibody reacts with natural and recombinant protein.

Imunogênio

Synthetic peptide (phosphorylated) corresponding to amino acids near the N-terminous of rat AANAT conjugated to BSA. {Ganguly, S et al, 2005}.

Aplicação

Anti-AANAT Antibody is an antibody against AANAT for use in WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Western Blot: 1:10,000-1:20,000 using ECL. Reacts with the ~23 kDa AANAT protein. Some reactivity may also be seen at ~97 kDa. Suggested blocking buffer is PBS with 10% non fat dry milk and 0.2% Tween-20. Suggested dilution buffer is PBS with 1 mg/mL BSA fraction V. Suggested incubation time is overnight at room temperature (~23°C).

Optimal working dilutions must be determined by the end user.

forma física

Liquid rabbit serum containing no preservative.

Armazenamento e estabilidade

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Nota de análise

Control
POSITIVE CONTROL:

Night adapted rat pineal gland. Expression is light sensitive; highest levels are obtained from animals at night.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Melatonin synthesis: 14-3-3-dependent activation and inhibition of arylalkylamine N-acetyltransferase mediated by phosphoserine-205.
Ganguly, Surajit, et al.
Proceedings of the National Academy of Sciences of the USA, 102, 1222-1227 (2005)
Andrzej Slominski et al.
European journal of biochemistry, 270(16), 3335-3344 (2003-08-06)
We showed expression of the tryptophan hydroxylase gene and of tryptophan hydroxylase protein immunoreactivity in mouse skin and skin cells. Extracts from skin and melanocyte samples acetylated serotonin to N-acetylserotonin and tryptamine to N-acetyltryptamine. A different enzyme from arylalkylamine N-acetyltransferase
Dynamics in enzymatic protein complexes offer a novel principle for the regulation of melatonin synthesis in the human pineal gland.
Erik Maronde,Anastasia Saade,Katrin Ackermann,Hany Goubran-Botros,Cecile Pagan,Roman Bux et al.
Journal of Pineal Research null
Jinglin Zhang et al.
Journal of pineal research, 65(1), e12481-e12481 (2018-02-27)
Melatonin is a key hormone that regulates circadian rhythms, metabolism, and reproduction. However, the mechanisms of melatonin synthesis and secretion have not been fully defined. The purpose of this study was to investigate the functions of the LIM homeobox transcription
Placental melatonin production and melatonin receptor expression are altered in preeclampsia: new insights into the role of this hormone in pregnancy.
Dave Lanoix,Pascale Guerin,Cathy Vaillancourt
Journal of Pineal Research null

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