AB3843

Anti-Ezrin/Radixin/Moesin Antibody

Chemicon^®, from rabbit

• Sinônimo(s): Anti-EBP50, Anti-NHERF, Anti-NHERF-1, Anti-NHERF1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: mouse, monkey, rat, human
• fabricante/nome comercial: Chemicon^®
• técnica(s): immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• nº de adesão NCBI: NM_004252.2
• nº de adesão UniProt: O14745
• Condições de expedição: dry ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... SLC9A3R1(9368)

Descrição

Especificidade

Recognizes Ezrin/Radixin/Moesin. These proteins function as linkers between the plasma membrane and the actin cytoskeleton, and are involved in cell adhesion, membrane ruffling, and microvilli formation. This antibody does not cross-react with other related proteins such as merlin or band 4.1.

Imunogênio

KLH-conjugated, synthetic peptide corresponding to residues surrounding Thr567 of human ezrin.

Aplicação

Detect Ezrin/Radixin/Moesin using this Anti-Ezrin/Radixin/Moesin Antibody validated for use in WB, IC, IH(P).

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Western blotting: 1:1,000. The 75 kDa band is Moesin and the 80kDa band is Ezrin and Radixin. (For best results, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight)

Immunohistochemistry (Paraffin): 1:50. Positive control tissue is breast carcinoma. Cellular localization is membrane and cytoplasm

Immunocytochemistry (IF): 1:100

Optimal working dilutions must be determined by the end user.

forma física

Protein A and peptide affinity purified immunoglobulin. Liquid in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, with 50% glycerol.

Armazenamento e estabilidade

Maintain at -20ºC for 12 months, do not aliquot.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2