AB1786P
Anti-Dopamine D3 Receptor Antibody, cytoplasmic domain
Chemicon®, from rabbit
Sinônimo(s):
D3R
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
rabbit
Nível de qualidade
forma do anticorpo
affinity purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
purificado por
affinity chromatography
reatividade de espécies
mouse, rat
fabricante/nome comercial
Chemicon®
técnica(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
dry ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
mouse ... Drd3(13490)
rat ... Drd3(29238)
Especificidade
Specific for Dopamine Receptor D3. Rat Dopamine D3 receptor is a 446 aa, G-protein coupled and transmembrane receptor protein (Giros et al. 1990, 1991; Schmauss et al. 1993; Liu et al. 1994; Fu et al. 1995; Sokoloff et al. 1990; Livingstone et al. 1992). The immunogen peptide shows no significant homology with other dopamine receptors (D1, D2, D4 or D5).
SPECIES REACTIVITIES: The immunogen peptide shows sequence homology of 88% with mouse and 63% with human/monkey (12/19 aa) D3 receptors (Giros et al. 1990, 1991; Schmauss et al. 1993; Liu et al. 1994; Fu et al. 1995; Sokoloff et al. 1990; Livingstone et al. 1992). Antibody cross-reactivity with various species is not known.
SPECIES REACTIVITIES: The immunogen peptide shows sequence homology of 88% with mouse and 63% with human/monkey (12/19 aa) D3 receptors (Giros et al. 1990, 1991; Schmauss et al. 1993; Liu et al. 1994; Fu et al. 1995; Sokoloff et al. 1990; Livingstone et al. 1992). Antibody cross-reactivity with various species is not known.
Imunogênio
A 19 amino acid peptide sequence from the rat D3 receptor within the 3rd cytoplasmic domain.
Epitope: cytoplasmic domain
Aplicação
Anti-Dopamine D3 Receptor Antibody, cytoplasmic domain detects level of Dopamine D3 Receptor & has been published & validated for use in ELISA, IH & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Western blot: 1-10 μg/mL (Chemiluminescence technique; 45-50 kDa) Approximately 100-400 μg of membrane protein/lane should be loaded to visualize the receptor band. In some systems 70kDa bands may also be present.
Immunohistochemistry: suggested working concentration is 2-10 μg/mL. Suggested fixative is 4% PFA, fixation 10-15′, permeabilized with 0.3-0.5% triton X-100 for 10-15′ (tissue, less time for cels), primary antibody dilution is 1:100 for direct fluorescence . {Coronas, V, et al. J Neurochemistry 91:1292-1301, 2004},
ELISA: 0.5-1.0 μg/mL (1 μg/mL immunogen peptide (Catalog Number AG229)/well)
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: suggested working concentration is 2-10 μg/mL. Suggested fixative is 4% PFA, fixation 10-15′, permeabilized with 0.3-0.5% triton X-100 for 10-15′ (tissue, less time for cels), primary antibody dilution is 1:100 for direct fluorescence . {Coronas, V, et al. J Neurochemistry 91:1292-1301, 2004},
ELISA: 0.5-1.0 μg/mL (1 μg/mL immunogen peptide (Catalog Number AG229)/well)
Optimal working dilutions must be determined by the end user.
forma física
Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA.
Armazenamento e estabilidade
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Moderate Alcohol Exposure during the Rat Equivalent to the Third Trimester of Human Pregnancy Alters Regulation of GABAA Receptor-Mediated Synaptic Transmission by Dopamine in the Basolateral Amygdala.
Diaz, MR; Jotty, K; Locke, JL; Jones, SR; Valenzuela, CF
Frontiers in pediatrics null
Dopamine D3-like receptors modulate anxiety-like behavior and regulate GABAergic transmission in the rat lateral/basolateral amygdala.
Diaz, MR; Chappell, AM; Christian, DT; Anderson, NJ; McCool, BA
Neuropsychopharmacology null
B Giros et al.
Biochemical and biophysical research communications, 176(3), 1584-1592 (1991-05-15)
Using Polymerase Chain Reaction amplification of mRNAs from several areas of rat brain we have shown the occurrence of two shorter transcripts of the dopamine D3 receptor gene, in addition to that corresponding to the D3 receptor. Cloning and sequencing
Vito S Hernández et al.
Neuropharmacology, 82, 88-100 (2013-12-03)
L-Dopa is the major symptomatic therapy for Parkinson's disease, which commonly occurs in elderly patients. However, the effects of chronic use on mood and cognition in old subjects remain elusive. In order to compare the effects of a chronic pulsatile
C D Livingstone et al.
The Biochemical journal, 287 ( Pt 1), 277-282 (1992-10-01)
Three-dimensional computer models of the rat D2, D3 and D4 dopamine receptor subtypes have been constructed based on the diffraction co-ordinates for bacteriorhodopsin, another membrane-bound protein containing seven transmembrane domains presumed to be arranged in a similar spatial orientation. Models
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