AB1076
Anti-phospho-ILK (Ser246) Antibody
from rabbit, purified by affinity chromatography
Sinônimo(s):
integrin-linked kinase, 59 kDa serine/threonine-protein kinase
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
rabbit
Nível de qualidade
forma do anticorpo
affinity isolated antibody
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
purificado por
affinity chromatography
reatividade de espécies
human, rat, mouse
reatividade da espécie (prevista por homologia)
ox (based on 100% sequence homology)
técnica(s)
immunofluorescence: suitable
western blot: suitable
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
phosphorylation (pSer246)
Informações sobre genes
human ... ILK(3611)
Descrição geral
ILK (Integrin-Linked Kinase) is an intraceullar Ser/Thr kinase with four Ankyrin-like repeats. It is known to play pivotal roles in extracellular matrix mediated signaling. ILK is known to associate with β-Integrins in addition to many other focal adhesion associated proteins. In this context, ILK is involved cell adhesion and migration. The kinase activity appears to be reduced following integrin activation, and overexpression of p59 ILK inhibits cellular adhesion to integrin substrates. Additionally, ILK has been associated with epithelial mesechymal transition (EMT) in a TGF-β1 induced Smad dependent manner (Li, 2003). Forced expression of ILK also induced the expression of MMP-2 (matrix metalloprotease 2) and promoted cell migration and invasion (Li, 2003).
Especificidade
This antibody recognizes ILK when phosphorylated at Ser246.
Imunogênio
Epitope: Phosphorylated Ser246
KLH-conjugated linear peptide corresponding to human ILK phosphorylated at Ser246.
Aplicação
Detect phospho-ILK (Ser246) using this Anti-phospho-ILK (Ser246) Antibody validated for use in WB & IF.
Immunofluorescence Analysis: A previous lot was used by an independent laboratory in BT549 breast cancer cells. (Dr. Shoukat Dedhar, BC Cancer Research Centre, Vancouver, BC, Canada.)
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Qualidade
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected ILK in 10 µg of HeLa cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected ILK in 10 µg of HeLa cell lysate.
Descrição-alvo
~54 kDa observed
forma física
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Armazenamento e estabilidade
Stable for 1 year at 2-8°C from date of receipt.
Nota de análise
Control
HeLa cell lysate
HeLa cell lysate
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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