70608

pETBlue-1 DNA - Novagen

Novagen′s pETBlue-1 vector is designed to identify recombinants by traditional blue/white screening while also allowing T7lac promoter based expression of target genes.

• Código UNSPSC: 41106610
• NACRES: NA.51

Propriedades

• Nível de qualidade: 100
• forma: liquid
• fabricante/nome comercial: Novagen^®
• condição de armazenamento: OK to freeze; avoid repeated freeze/thaw cycles
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

The pETBlue-1 vector is designed to identify recombinants by traditional blue/white screening while also allowing T7lac promoter based expression of target genes. Screening is independent of expression because the T7lac expression promoter is in an opposed orientation relative to the E. coli promoter that mediates blue/white screening. pETBlue-1 facilitates the expression of native unfused proteins and allows convenient subcloning of target genes already fused to existing detection and purification tags. The EcoR V cloning site is appropriately spaced downstream of an E. coli ribosome binding site. Inserts must encode an ATG start codon at their 5′ end if expression is desired. The sequence is numbered from the first base of the T7 promoter sequence.

Advertência

Toxicity: Standard Handling (A)

Informações legais

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable