681660-M

N-WASP-Inhibitor, 187-1

The N-WASP-Inhibitor, 187-1 controls the biological activity of neural Wiskott Aldrich syndrome protein (N-WASP). This small molecule/inhibitor is primarily used for Cell Structure applications.

• Sinônimo(s): N-WASP-Inhibitor, 187-1, cyclo( LK DF DP DF LF DP LQ)₂
• Fórmula empírica (Notação de Hill): C₉₆H₁₂₂N₁₈O₁₆
• Peso molecular: 1784.11
• Código UNSPSC: 12352200

Propriedades

• Nível de qualidade: 100
• Ensaio: ≥95% (HPLC)
• Formulário: lyophilized solid
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; desiccated (hygroscopic)
• cor: white
• solubilidade: DMSO: 1 mg/mL; acetic acid: 5%
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

A 14-amino acid cyclic peptide that potently blocks phosphatidylinositol 4,5-bisphosphate (PIP₂)-induced actin assembly in Xenopus egg cytoplasmic extract (IC₅₀ ~ 2 µM). Shown to directly bind to neural Wiskott Aldrich syndrome protein (N-WASP) with high-affinity, and prevent the activation of actin-related protein 2/3 (Arp2/3) complex by inhibiting the interaction between N-WASP and Arp2/3.

A 14-amino acid cyclic peptide that potently blocks phosphatidylinositol 4,5-bisphosphate (PIP₂)-induced actin assembly in Xenopus egg cytoplasmic extract (IC₅₀ ~2 µM). Shown to directly bind to neural Wiskott Aldrich syndrome protein (N-WASP) with high-affinity, and allosterically stabilize the autoinhibited conformation of N-WASP. It prevents the activation of actin-related protein 2/3 (Arp2/3) complex by blocking the interaction between N-WASP and Arp2/3.

Ações bioquímicas/fisiológicas

Primary Target
Neural Wiskott Aldrich syndrome protein (N-WASP)

Target IC₅₀: ~ 2 µM blocking phosphatidylinositol 4,5-bisphosphate (PIP2)-induced actin assembly in Xenopus egg cytoplasmic extract

Advertência

Toxicity: Standard Handling (A)

Sequência

cyclo(LLys-DPhe-DPro-DPhe-LPhe-DPro-LGln)₂

forma física

Supplied as a trifluoroacetate salt.

Reconstituição

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Outras notas

Peterson, J.R., et al. 2001. Proc. Natl. Acad. Sci. USA98, 10624.
Suetsugu, S., et al. 2001. J. Biol. Chem.276, 33175.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable