598101

Thioredoxin, Human, Recombinant, E. coli

• Sinônimo(s): Thioredoxin, Human, Recombinant, E. coli, rhTRX
• Número CAS: 52500-60-4
• Número MDL: MFCD00132423
• Código UNSPSC: 12352202
• NACRES: NA.77

Propriedades

• recombinante: expressed in E. coli
• Nível de qualidade: 100
• Ensaio: ≥90% (SDS-PAGE)
• Formulário: lyophilized solid
• atividade específica: ≥1.7 units/mg protein
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• Condições de expedição: ambient
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Recombinant, human thioredoxin expressed in E. coli. A ubiquitous protein with two redox-active half-cysteine residues that function as a general disulfide reductant. Works efficiently on model compounds (e.g. Ellman′s reagent, Cat. No. 322123) and protein disulfides. Plays an essential role in cellular protection against oxidative stress and cell death. Inhibits apoptosis in Jurkat T-cells and human PBL blasts. Occurs as a single polypeptide chain.

Recombinant, human thioredoxin expressed in E. coli. A ubiquitous protein with two redox-active half-cysteine residues. Functions as a general disulfide reductant. Works efficiently on model compounds (e.g. Ellman’s reagent, Cat. No. 322123) and protein disulfides. Plays an essential role in cellular protection against oxidative stress and cell death. Inhibits apoptosis in Jurkat T cells and human PBL.

Advertência

Toxicity: Standard Handling (A)

Definição da unidade

One unit (insulin reduction unit) is calculated by measuring the change in absorbance at 650 nm with 330 µM DTT as the reductant at 25°C, pH 6.5.

Reconstituição

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Reconstitute in physiological buffer containing 3 mM EDTA and 1-5 mM DTT.

Outras notas

Noda, N., et al. 1997. Nippon Rinsho 55, 461.
Wollman, E.E., et al. 1997. Biochem. Biophys. Res. Commun. 230, 602.
Masutani, H., et al. 1996. Immunol. Lett. 54, 67.
Saito, I., et al. 1996. Arthritis Rheumatism, 39, 773.
Saito, N., et al. 1995. J. Immunol. 154, 3194.
Buchanon, B.B., et al. 1994. Arch. Biochem. Biophys. 314, 257.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable