598502

Thioredoxin Reductase, Yeast, Recombinant, E. coli

• Sinônimo(s): ryNTR
• Código UNSPSC: 12352202
• NACRES: NA.54

Propriedades

• recombinante: expressed in E. coli
• Nível de qualidade: 100
• Ensaio: ≥95% (SDS-PAGE)
• Formulário: lyophilized solid
• atividade específica: ≥5 units/mg protein
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• solubilidade: aqueous buffer, pH 7.0: soluble
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Recombinant, yeast thioredoxin reductase expressed in E. coli. Yeast thioredoxin reductase is a flavoprotein that has no selenocysteine (SeCys). It catalyzes the NADPH-dependent reduction of thioredoxin as well as other endogenous and exogenous proteins. Plays an important role in many aspects of cell function, including protection against oxidative stress/injury, cell growth and transformation, and the recycling of ascorbate from its oxidized form.

Advertência

Toxicity: Standard Handling (A)

Definição da unidade

One unit is defined as the amount of enzyme that will cause an absorbance change of 1 at 412 nm using 200 µM NADPH and 5 mM DTNB, per min at 25°C, pH 7.0.

forma física

Lyophilized from 150 mM NaCl, 20 mM phosphate buffer, pH 7.4.

Reconstituição

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for 1 month at 4°C or 3 months at -70°C.

Outras notas

Mustacich, D. and Powis, G. 2000. Biochem. J.346, 1.
Wang, P.F., et al. 1998. Biochemistry37, 16378.
Holmgren, A., 1979. J. Biol. Chem. 254, 9627.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable