495609

Okadaic Acid, Prorocentrum sp.

InSolution, ≥95%, inhibitor of protein phosphatase 1

• Sinônimo(s): InSolution Okadaic Acid, Prorocentrum sp.
• Fórmula empírica (Notação de Hill): C₄₄H₆₈O₁₃
• Peso molecular: 805.00
• Código UNSPSC: 12352106

Propriedades

• Nível de qualidade: 100
• Ensaio: ≥95% (HPLC)
• forma: liquid
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; desiccated (hygroscopic); protect from light
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

An ionophore-like polyether derivative of a C₃₈ fatty acid compound isolated from dinoflagellates that have fed on the marine sponge Halinchrondria okadai. It is a potent non-comepetitive, reversible inhibitor of serine/threonine-specific protein phosphatases 1 (PP1, IC₅₀ = 10-15 nM, rabbit skeletal muscle, catalytic subunit) and 2A (PP2A, IC₅₀ = 0.1 nM, rabbit skeletal muscle, catalytic subunit). It has only a trivial effect on protein phosphatase 2B (IC₅₀ = 5 µM), a Ca²⁺/calmodulin-dependent enzyme, while PP2C, a Mg²⁺ dependent enzyme, is unaffected. Okadaic acid has no significant effect on the activities of tyrosine phosphatases, alkaline phosphatase, acid phosphatase, and inositol trisphosphatase. It is also a non-phorbol ester-type tumor promoter on mouse skin. Useful for the study of protein phosphatases and of PP1/PP2A in cell extracts, as well as in intact cells. Induces apoptosis in human breast carcinoma (MB-231 and MCF-7) and in myeloid cells, but inhibits glucocorticoid-induced apoptosis in T-cell hybridomas. Has marked contractile effect on smooth muscles and heart muscles. Implicated as a causative agent of diarrhetic shellfish poisoning.

Ações bioquímicas/fisiológicas

Cell permeable: no

Primary Target
protein phosphatase

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 10-15 nM against protein phosphatase 1; 0.1 nM against protein phosphatase 2A

Embalagem

Packaged under inert gas

Advertência

Toxicity: Toxic (F)

forma física

A 250 µM (25 µg/124 µl) solution of Okadaic Acid, (Cat. No. 495604) in DMSO.

Reconstituição

Following initial thaw, aliquot and freeze (-20°C).

Outras notas

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

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Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• Ponto de fulgor (°C): 87 °C - closed cup - (Dimethylsulfoxide)