475821

Microcystin-LR

from Microcystis aeruginosa, ≥95% (HPLC), liquid, protein phosphatase 2A and protein phosphatase 1 inhibitor, Calbiochem^®

• Sinônimo(s): InSolution Microcystin-LR, Microcystis aeruginosa
• Fórmula empírica (Notação de Hill): C₄₉H₇₄N₁₀O₁₂
• Peso molecular: 995.17
• Código UNSPSC: 12352200
• NACRES: NA.77

Propriedades

• Nome do produto: Microcystin-LR, Microcystis aeruginosa, InSolution, ≥95%, inhibitor of protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A)
• Nível de qualidade: 100
• Ensaio: ≥95% (HPLC)
• Formulário: liquid
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; desiccated (hygroscopic); protect from light
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Cyclic heptapeptide toxin isolated from the freshwater cyanobacteria, Microcystis aeruginosa. Potent inhibitor of protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A). Unlike Okadaic Acid (Cat. No. 495604), microcystin-LR is effective on both PP1 (IC₅₀ = 1.7 nM) and PP2A (IC₅₀ = 40 pM). Reacts with phosphatase in a two-step mechanism involving rapid binding and inactivation of the catalytic subunit followed by slower covalent interactions. Has no effect on protein kinases, making it useful for reducing contaminating phosphatase activities in protein kinase assays.

Ações bioquímicas/fisiológicas

Cell permeable: no

Primary Target
protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A)

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 1.7 nM, 40 pM, against protein phosphatase 1 (PP1), protein phosphatase 2A (PP2A), respectively

Embalagem

Packaged under inert gas

yes

Advertência

Toxicity: Highly Toxic (H)

forma física

A 2.5 mM (250 µg/100 µl) solution of Microcystin-LR, Microcystis aeruginosa (Cat. No. 475815) in DMSO.

Reconstituição

Following initial thaw, aliquot and freeze (-20°C).

Outras notas

Craig, M., et al. 1996. Biochem. Cell Biol. 74, 569.
Nishiwaki, R., et al. 1994. Cancer Lett. 83, 283.
Amick, G.D., et al. 1992. Biochem. J.287, 1019.
Davidson, H.W., et al. 1992. J. Cell Biol.116, 1343.
Nishiwaki-Matsushima, R., et al. 1992. J. Cancer Res. Clin. Oncol. 118, 420.
Cohen, P., et al. 1990. Trends Biochem. Sci.15, 98.
MacKintosh, C., et al. 1990. FEBS Lett.264, 187.
Cohen, P., et al. 1989. FEBS Lett.250, 596.
Carmichael, W.W. 1988. in Handbook of Natural Toxins3,Marine Toxins and Venoms (Tu, A.T., Ed.) p. 121, Decker, New York.

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Informações legais

Not available for sale outside of the United States.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• Ponto de fulgor (°C): 87 °C - closed cup - (Dimethylsulfoxide)